红芪多糖对内毒素诱导的大鼠葡萄膜炎TRAF6及TRIF表达的影响

发布时间：2018-12-30 11:35

【摘要】：目的:研究红芪多糖对内毒素诱导的大鼠葡萄膜炎TLR-4(Toll样受体-4)信号转导通路中下游信号转导通路元件TRAF6(肿瘤坏死因子受体相关因子-6)及TRIF(Toll样受体活化相关因子)表达的影响,探讨红芪多糖抑制内毒素诱导的大鼠葡萄膜炎可能的作用机制。方法:实验选用Wistar大鼠作为研究对象,采用霍乱弧菌内毒素1mg/kg皮下注射的方法,建立大鼠急性前葡萄膜炎动物模型。Wistar大鼠160只被随机分为4组,每组40只:空白对照组,EIU模型组,LPS+HPS组(内毒素+红芪多糖组)和HPS组(红芪多糖组)。LPS+HPS组及空白对照组大鼠分别在内毒素注射前1小时给予腹腔注射红芪多糖400 mg/kg及等量磷酸盐缓冲液。HPS组大鼠只注射红芪多糖400 mg/kg。每2小时用裂隙灯观察大鼠眼前节炎症反应并评分。组织病理学检查观察炎症反应水平。实时定量PCR检测TRAF6及TRIF m RNA的表达。用蛋白免疫印迹法从蛋白水平检测TRAF6及TRIF的表达。结果:成功建立急性前葡萄膜炎动物模型。LPS+HPS组大鼠眼前节炎症反应及组织病理学炎症表现明显减轻。实时定量PCR结果显示红芪多糖可抑制TRAF6m RNA的表达,抑制其蛋白的降解,红芪多糖对TRIF的作用不明显。结论:红芪多糖可以通过抑制TRAF6核酸的表达和蛋白的降解缓解内毒素诱导的大鼠急性前葡萄膜炎眼内炎症反应。红芪多糖对EIU模型的干预途径主要是通过阻断TLR4-My D88依赖途径而发挥的作用。
[Abstract]:Objective: to study the effects of hongqi polysaccharide on endotoxin-induced uveitis TLR-4 (Toll like receptor 4) signal transduction pathway element TRAF6 (tumor necrosis factor receptor related factor 6) and TRIF ( The expression of Toll like receptor activation-related factors, To explore the possible mechanism of hongqi polysaccharide inhibiting endotoxin induced uveitis in rats. Methods: Wistar rats were selected as the research object. The animal model of acute anterior uveitis was established by subcutaneous injection of vibrio cholerae endotoxin 1mg/kg. 160 Wistar rats were randomly divided into 4 groups, 40 rats in each group: blank control group. EIU model group, Rats in LPS HPS group (lipopolysaccharide group) and HPS group (Hongqi polysaccharide group). LPS HPS group and blank control group) were given intraperitoneal injection of Hongqi polysaccharide 400 mg/kg and the same amount of phosphate buffer 1 hour before endotoxin injection, respectively. Liquid. Rats in HPS group were injected with polysaccharides of Rhizoma membranaceus for 400 mg/kg.. The inflammatory reaction of rat anterior segment was observed by slit lamp every 2 hours. The level of inflammatory reaction was observed by histopathological examination. The expression of TRAF6 and TRIF m RNA was detected by real-time quantitative PCR. The expression of TRAF6 and TRIF was detected by Western blot at protein level. Results: the inflammatory reaction and histopathological inflammation of anterior uveitis in. LPS HPS group were alleviated obviously. The results of real-time quantitative PCR showed that the polysaccharides of Hongqi could inhibit the expression of TRAF6m RNA and the degradation of its protein, but the effect of polysaccharides on TRIF was not obvious. Conclusion: hongqi polysaccharide can alleviate the intraocular inflammation induced by endotoxin in rats with acute anterior uveitis by inhibiting the expression of TRAF6 nucleic acid and protein degradation. The intervention pathway of APS on EIU model was mainly by blocking the TLR4-My D88 dependent pathway.
【学位授予单位】：首都医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R773

【参考文献】