紫外线照射对大鼠泪膜稳定性和角膜组织的影响

发布时间：2018-12-30 17:21

【摘要】：目的探究不同强度和时间紫外线照射对大鼠泪膜稳定性和角膜组织的影响。设计实验研究。研究对象50只健康SD大鼠(6周,200~250 g)。方法将大鼠随机分成5组(空白对照组N和实验组A、B、C、D),每组10只,A、C组紫外线照射强度为142μw/cm~2,每次照射时长为8、12分钟;B、D紫外线照射强度为94μw/cm~2,每次照射时长为12、18分钟。共照射42天,每周照射3次,均间隔1天,照射后第14、28、42天,裂隙灯检查、荧光素染色及泪膜破裂时间(BUT)测定、Schirmer I试验(SIt)。49天时注射过量水合氯醛处死大鼠,制作角膜组织切片。主要指标结膜、角膜、泪膜形态结构变化,角膜荧光素染色评分,BUT,SIt值,角膜组织切片观察。结果裂隙灯检查显示B、C组对比,照射强度较高组比较低组睑结膜充血、角膜水肿、角膜混浊出现早且严重,较高强度组照射后出现角膜新生血管,较低强度组以泪液质和量改变为主要特征。A、C组对比,长时间照射组比短时间照射组角膜水肿、混浊和新生血管出现的时间早且严重,且长时间照射组睑裂区出现条状溃疡。荧光素染色显示,正常组染色评价结果为阴性,A组为阳性,B组为弱阳性,C、D组为强阳性。第42天,A、B、C、D组BUT值分别较对照组明显减少(P均0.01)。第42天,实验组SIt值分别较对照组均明显增加(P均0.01)。组织学观察显示,较高强度紫外线照射引起角膜新生血管,内皮细胞密度下降,基质层纤维细胞排列紊乱。较低强度短时间照射引起上皮层增厚。较低强度长时间照射引起角膜新生血管,基质层纤维细胞排列紊乱。结论长时间紫外线照射可引起睑结膜充血、角膜上皮损伤、泪液质和量改变等眼表结构变化,这些变化随照射强度增大或照射时间增长而出现更早、程度更重。
[Abstract]:Objective to investigate the effects of ultraviolet irradiation on tear film stability and corneal tissue in rats. Design experimental research. Participants 50 healthy SD rats (6 weeks, 200,250 g).) Methods the rats were randomly divided into 5 groups (control group N and experimental group Acarinic D) with 10 rats in each group. The ultraviolet radiation intensity was 142 渭 w / cm ~ (-2) in APC group, and the duration of each irradiation was 812 minutes. The intensity of ultraviolet radiation was 94 渭 w / cm ~ (-2), and the duration of each irradiation was 12 ~ (18) minutes. The rats were killed by slit lamp examination, fluorescein staining and lacrimal film rupture time (BUT). The rats were killed by injection of excessive chloral hydrate on day 49 of, Schirmer I test (SIt). 49. Corneal tissue sections were made. Main outcome measures: changes in morphology and structure of conjunctiva, cornea and lacrimal film, corneal fluorescein staining score, BUT,SIt value and corneal tissue sections. Results slit-lamp examination showed that the blepharoconjunctival congestion, corneal edema and corneal opacity appeared earlier and more seriously in the higher radiation intensity group than in the lower group, and corneal neovascularization appeared in the higher intensity group after irradiation. The changes of tear quality and quantity were the main characteristics in the low intensity group. Compared with the control group, the corneal edema, turbidity and neovascularization appeared earlier and more seriously in the long time irradiation group than in the short time irradiation group. In the long-term irradiation group, stripe ulcers appeared in the cleft eyelid region. The results of fluorescein staining were negative in normal group, positive in group A, weak positive in group B and strong positive in group C and D. On the 42nd day, the BUT values of the D group were significantly lower than those of the control group (P 0.01). On the 42 th day, the SIt value of the experimental group was significantly higher than that of the control group (P 0.01). Histopathological observation showed that the corneal neovascularization was induced by high intensity ultraviolet irradiation, the density of endothelial cells decreased, and the fibroblasts of stromal layer were arranged in disorder. The thickening of the epithelium was induced by low intensity and short time irradiation. Low intensity and long time irradiation resulted in corneal neovascularization and disorder in the arrangement of fibroblasts in the stromal layer. Conclusion Ultraviolet irradiation for a long time can cause hyperemia of eyelid conjunctiva, corneal epithelium injury, tear quality and quantity change. These changes appear earlier and more serious with the increase of irradiation intensity or irradiation time.
【作者单位】：福建医科大学医学技术与工程学院眼视光学系;
【基金】：福建医科大学2015年国家级大学生创新创业训练计划项目(201510392026) 福建省自然科学基金(2016J01155)
【分类号】：R77

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