MicroRNA-181a在各型OSAHS患者血清中表达情况的研究
发布时间：2019-01-30 09:14
【摘要】：[目的]通过检测不同患病程度的OSAHS患者外周血清中microRNA-181a浓度,分析其与多导睡眠监测指标的相关性,探讨microRNA-181a的表达与OSAHS患者的关系。[方法]1、收集2016年9月至2017年1月期间前往昆明医科大学第一附属医院头颈鼾症中心行多导睡眠监测(Polysomnography,PSG)的男性患者41例,年龄35～65岁。根据中华医学会OSAHS诊治指南PSG结果进行分组,其中轻度OSAHS组20例,中、重度OSAHS组21例。同时选取同时期进行体检的健康男性19名作为对照组。采集所有受试者基本信息及PSG检查结果,均排除慢性肺部病变、高血压及其他心脑血管疾病、糖尿病及其他内分泌疾病、甲状腺疾病、营养代谢疾病。既往无特殊药物使用史,睡眠监测前一周内无上呼吸道感染或发热史,戒烟戒酒≥1周,所有患者均知情同意。2、受试者取晨起空腹血样3ml,血液样品离体后立即置于无DNA酶和RNA酶的灭菌采血管中,自然静置,800×g离心5min后采集血清,-80℃保存备用。3、血清RNA提取:取上述血清样本,按照miRcute miRNA提取试剂盒说明书所述的方法提取血清RNA。采用紫外分光光度法测定RNA浓度。4、miRNA逆转录合成cDNA,Q-PCR扩增目的基因检测miRNA水平。5、收集结果,并进行数据分析及统计学处理。[结果]1、轻度OSAHS组患者血清中miR-18 1a表达水平(10.5±0.5)与正常组(10.2±0.1)相比,差异无统计学意义(P0.05);但中、重度OSAHS组患者血清中miR-181a表达水平(12.8±1.4)较正常组明显升高,且差异有统计学意义(P0.05)。组间比较显示,中、重度OSAHS组患者血清中miR-181a表达水平较轻度OSAHS组患者升高,且差异有统计学意义(P0.05)。提示OSAHS患者血清中miR-181a表达水平高于正常人,且中、重度OSAHS患者血清中miR-181a表达水平较轻度OSAHS患者升高(F=1 6.622,P0.001)。2、OSAHS患者血清中miR-181a表达水平与AHI、TS90%相关性分析均呈正相关(r值分别为0.752和0.778,均P0.01)。3、OSAHS患者血清中miR-181a表达水平与LSa02水平相关性分析呈负相关(r值为-0.388,P0.01)。[结论]1、随OSAHS程度加重,miR-181a表达水平升高;随AHI升高、TS90%增大,miR-181a表达水平升高;随LSa02水平降低,miR-181a表达水平升高。2、miR-181a与OSAHS患者PSG各指标密切相关,两者联合应用能进一步提高对中、重度OSAHS的诊断效能及指导治疗,同时协助评估CPAP的治疗效果。
[Abstract]:[objective] to investigate the relationship between microRNA-181a expression and polysomnography in patients with OSAHS. [methods] from September 2016 to January 2017, 41 male patients, aged 35 or 65 years, who went to the head and neck snoring Center of the first affiliated Hospital of Kunming Medical University for polysomnography (Polysomnography,PSG) were collected. According to the PSG results of OSAHS diagnosis and treatment guidelines of the Chinese Medical Association, there were 20 cases in mild OSAHS group, 21 cases in moderate and severe OSAHS group. At the same time, 19 healthy men who underwent physical examination at the same time were selected as the control group. The basic information of all subjects and the results of PSG examination were all excluded from chronic pulmonary diseases, hypertension and other cardiovascular and cerebrovascular diseases, diabetes and other endocrine diseases, thyroid diseases and nutritional and metabolic diseases. There was no history of special drug use, no history of upper respiratory tract infection or fever within one week before sleep monitoring, smoking and alcohol cessation 鈮，
本文编号：2417982
[Abstract]:[objective] to investigate the relationship between microRNA-181a expression and polysomnography in patients with OSAHS. [methods] from September 2016 to January 2017, 41 male patients, aged 35 or 65 years, who went to the head and neck snoring Center of the first affiliated Hospital of Kunming Medical University for polysomnography (Polysomnography,PSG) were collected. According to the PSG results of OSAHS diagnosis and treatment guidelines of the Chinese Medical Association, there were 20 cases in mild OSAHS group, 21 cases in moderate and severe OSAHS group. At the same time, 19 healthy men who underwent physical examination at the same time were selected as the control group. The basic information of all subjects and the results of PSG examination were all excluded from chronic pulmonary diseases, hypertension and other cardiovascular and cerebrovascular diseases, diabetes and other endocrine diseases, thyroid diseases and nutritional and metabolic diseases. There was no history of special drug use, no history of upper respiratory tract infection or fever within one week before sleep monitoring, smoking and alcohol cessation 鈮，
本文编号：2417982
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