FOXO3a和HIF-1α在鼻咽癌中的表达及其临床意义

发布时间：2019-02-26 15:10

【摘要】：目的：鼻咽癌（Nasopharyngealcarcinoma,NPC）是我国最常见的头颈部肿瘤。由于鼻咽癌发生部位隐蔽、早起症状轻微，约60%的病人在确诊为鼻咽癌时已发生淋巴结转移或远处转移。因此，研究鼻咽癌的发生机制并寻找新的诊断和判断分子标记物具有重要意义。研究发现，FOXO3a转录因子和缺氧诱导因子HIF-1α在许多人类肿瘤的发展和预后中起重要的作用。然后，其具体作用并不清楚。因此，，我们研究FOXO3a和HIF-1α在鼻咽癌中的表达水平和临床意义，为鼻咽癌治疗提供新的策略。方法：应用免疫组化、RT-PCR和Westernblot法检测48例鼻咽癌组织和15例正常鼻咽粘膜组织中FOXO3a和HIF-1α的mRNA、蛋白的表达水平，分析FOXO3a和HIF-1α与各种临床病理参数之间的关系。结果：FOXO3a蛋白在鼻咽癌组织中的表达率（50.0%）明显低于正常鼻咽粘膜组织中的表达率（80.0%），差异明显，且统计学意义（P=0.006）。而HIF-1α蛋白在鼻咽癌中的表达率（75.0%）明显高于正常鼻咽粘膜组织中的表达率（33.3%），差异有统计学意义（P=0.011）。FOXO3a蛋白的表达与鼻咽癌的临床分期、T分期、淋巴结转移、远处转移密切相关（P0.05）,HIF-1α蛋白的表达与鼻咽癌的淋巴结转移、远处转移密切相关（P0.05）。FOXO3a和HIF-1αmRNA在鼻咽癌和正常鼻咽粘膜组织中的表达差异无统计学意义。Spearman相关分析结果显示FOXO3a和HIF-1α蛋白的表达呈负相关（rs= 0.667,P0.001）。结论：FOXO3a和HIF-1α的异常表达可能与鼻咽癌的肿瘤生成、发展过程有一定的联系。FOXO3a和HIF-1α可能成为判断鼻咽癌生物学行为的重要指标。
[Abstract]:Objective: nasopharyngeal carcinoma (Nasopharyngealcarcinoma,NPC) is the most common head and neck tumor in China. Due to the occult location of nasopharyngeal carcinoma (NPC) and mild symptoms of early onset, about 60% of the patients had lymph node metastasis or distant metastasis at the time of diagnosis of nasopharyngeal carcinoma (NPC). Therefore, it is of great significance to study the pathogenesis of nasopharyngeal carcinoma and to find new molecular markers. It has been found that FOXO3a transcription factor and hypoxia inducible factor HIF-1 伪 play an important role in the development and prognosis of many human tumors. Then, its specific role is not clear. Therefore, we study the expression level and clinical significance of FOXO3a and HIF-1 伪 in nasopharyngeal carcinoma, and provide a new strategy for the treatment of nasopharyngeal carcinoma. Methods: the expression of mRNA, protein of FOXO3a and HIF-1 伪 in 48 cases of nasopharyngeal carcinoma and 15 cases of normal nasopharyngeal mucosa were detected by immunohistochemistry, RT-PCR and Westernblot methods. The relationship between FOXO3a and HIF-1 伪 and various clinicopathological parameters was analyzed. Results: the expression rate of FOXO3a protein in nasopharyngeal carcinoma (50. 0%) was significantly lower than that in normal nasopharyngeal mucosa (80. 0%), and the difference was statistically significant (P < 0. 006). The expression rate of HIF-1 伪 protein in nasopharyngeal carcinoma (75.0%) was significantly higher than that in normal nasopharyngeal mucosa (33.3%), and the difference was statistically significant (P < 0.011). The expression of FOXO3a protein was related to the clinical stage of nasopharyngeal carcinoma (NPC). T stage, lymph node metastasis and distant metastasis were closely correlated (P0.05). The expression of HIF-1 伪 protein was associated with lymph node metastasis of nasopharyngeal carcinoma. There was no significant difference in the expression of FOXO3a and HIF-1 伪 mRNA between nasopharyngeal carcinoma and normal nasopharyngeal mucosa. The results of Spearman correlation analysis showed that the expression of FOXO3a and HIF-1 伪 protein was negatively correlated with the expression of FOXO3a and FOXO3a in nasopharyngeal carcinoma (NPC) and normal nasopharyngeal mucosa (P < 0. 667, P < 0. 05). P0.001). Conclusion: the abnormal expression of FOXO3a and HIF-1 伪 may be related to the tumorigenesis and development of nasopharyngeal carcinoma. FOXO3a and HIF-1 伪 may be important markers to judge the biological behavior of nasopharyngeal carcinoma.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.63

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