HMGA1与Ezrin在喉癌组织中的表达及相关性研究

发布时间：2019-03-12 12:26

【摘要】：目的：探讨高迁移蛋白A1（high mobility group protein A1,HMGA1）与埃兹蛋白（Ezrin）在喉癌组织中的表达及关系。方法：采用免疫组织化学（SP法）染色方法检测HMGA1、Ezrin蛋白在50例喉癌组织、30例喉不典型增生组织、30例喉息肉中的表达；采用实时定量PCR法测定法检测HMGA1、Ezrin mRNA在30例新鲜喉癌组织及30例癌切缘组织的表达；Western Blot方法检测HMGA1蛋白及Ezrin蛋白在30例新鲜喉癌组织及30例癌切缘组织的表达，分别统计分析二者表达与临床病理特征的关系及相关性。结果：(1)HMGA1在喉息肉、喉不典型增生组织和喉癌组织的表达阳性率分别为13.0%(4/30)、53.0%(16/30)和68.0%(34/50)，三组比较有统计学差异(P 0.05，表2)。HMGA1蛋白表达与喉癌组织的临床分期、组织学分级及转移密切相关(P0.05,表3)。Ezrin蛋白在对应三组阳性率分别为23.3%(7/30)，50.0%(15/30)和64.0%(32/50)，三组比较有统计学差异(P 0.05)。(2)实时定量PCR示：在30例喉癌组织（100%）以及配对癌切缘组织（100%）中均检测到HMGA1的表达，两者在喉癌组织相对于癌切缘组织的表达量具有显著差异（HMGA1: P 0.01;Ezrin: P 0.05)。(3) Western Blot提示：在30例喉癌组织（100%）以及配对癌切缘组织（100%）中均检测到HMGA1蛋白及Ezrin蛋白的表达，两者在喉癌组织相对于癌切缘组织的表达量具有显著差异，（HMGA1, P 0.01;Ezrin, P0.01)，单因素方差分析示，HMGA1、Ezrin与组织学分级（P 0.05）、淋巴结转移（P＜0.05）、临床分期（P 0.05）及淋巴结转移有关（P 0.05）有关，而与吸烟、年龄及性别无关(P0.05)。(4)喉癌组织中HMGA1及Ezrin蛋白、mRNA水平表达正相关(实时定量PCR：r=0.385，P=0.000；免疫组织化学：x2=7.172，r=0.379，P=0.012；Western blot：r=0.412，，P=0.000)。结论：（1）HMGA1和Ezrin蛋白及mRNA在喉癌组织中表达异常，其表达量高于正常喉上皮组织；在喉不典型增生恶性转化过程中，随病变程度增加，HMGA1、Ezrin表达阳性率逐渐增加；（2）HMGA1和Ezrin蛋白及mRNA与淋巴结转移、病理分级和临床分期相关，与患者的年龄、性别、肿瘤的部位无明显相关，两者在喉癌组织的表达存在正相关；（3） HMGA1和Ezrin在喉癌组织中的表达增高，在喉癌的发生、发展过程中可能起协同作用，可作为喉癌预测转移和判断预后的生物学标志物。
[Abstract]:Aim: to investigate the expression and relationship between high migration protein A1 (high mobility group protein A1 (HMG A1) and Ezrin (Ezrin) in laryngeal carcinoma. Methods: immunohistochemical staining (SP method) was used to detect the expression of HMGA1,Ezrin protein in 50 cases of laryngeal carcinoma, 30 cases of laryngeal dysplasia and 30 cases of laryngeal polyps. Real-time quantitative PCR was used to detect the expression of HMGA1,Ezrin mRNA in 30 fresh laryngeal carcinoma tissues and 30 incisor margin tissues. Western Blot method was used to detect the expression of HMGA1 protein and Ezrin protein in 30 fresh laryngeal carcinoma tissues and 30 incisor margin tissues. The correlation between the expression and clinicopathological features was analyzed statistically. Results: (1) the positive rates of HMGA1 expression in laryngeal polyp, laryngeal dysplasia and laryngeal carcinoma were 13.0% (4 / 30), 53.0% (16 / 30) and 68.0% (34 / 50), respectively. The expression of HMGA1 protein was correlated with the clinical stage, histological grade and metastasis of laryngeal carcinoma (P 0.05, P < 0.01). The positive rates of Ezrin protein in the corresponding three groups were 23.3% (7 / 30), 50.0% (15 / 30) and 64.0% (32 / 50), respectively. There was statistical difference among the three groups (P0.05). (2). The expression of HMGA1 was detected in 30 cases of laryngeal carcinoma (100%) and matched adjacent tissues (100%), and the expression of PCR was detected in 30 cases of laryngeal carcinoma (100%) and in matched adjacent tissues (100%). There was a significant difference in the expression of HMGA1: P 0.01 between laryngeal carcinoma and tumor incisors (P < 0.01). Ezrin: P 0.05). (3) Western Blot showed that the expression of HMGA1 protein and Ezrin protein were detected in 30 cases of laryngeal carcinoma (100%) and in matched adjacent tissues (100%), and the expression of HMGA1 protein and Ezrin protein were detected in 30 cases of laryngeal carcinoma (100%). There was a significant difference in the expression of HMGA1, P 0.01 between laryngeal carcinoma and the incisor margin of carcinoma (P < 0.01). Ezrin, (P0.01), one-way ANOVA analysis showed that HMGA1,Ezrin was related to histological grade, lymph node metastasis, clinical stage and lymph node metastasis, but was related to smoking, but correlated with the histological grade (P 0.05), lymph node metastasis (P < 0.05), clinical stage (P 0.05) and lymph node metastasis (P 0.05). There was no significant difference between age and sex (P0.05). (4). There was a positive correlation between the expression of HMGA1, Ezrin protein and mRNA (real time quantitative PCR:r=0.385,P=0.000;) in laryngeal carcinoma. Immunohistochemical staining: x2 / 7.172, r = 0.379, P = 0.012 / Western blot:r=0.412,P=0.000). Conclusion: (1) the expression of HMGA1, Ezrin and mRNA in laryngeal carcinoma is abnormal, which is higher than that in normal laryngeal epithelial tissue, and the positive rate of HMGA1,Ezrin expression gradually increases with the degree of malignant transformation of laryngeal dysplasia. (2) the expression of HMGA1, Ezrin and mRNA were positively correlated with lymph node metastasis, pathological grade and clinical stage, but not with age, sex or tumor location. (3) the increased expression of HMGA1 and Ezrin in laryngeal carcinoma may play a synergistic role in the carcinogenesis and development of laryngeal carcinoma, which can be used as biomarkers for predicting metastasis and predicting prognosis of laryngeal carcinoma.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.65

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