纤维蛋白胶羊膜棒在去势雄兔干眼症的应用研究

发布时间：2019-03-19 07:50

【摘要】：目的研究纤维蛋白胶羊膜棒对去势雄兔干眼症的预防和治疗作用。方法选取雄性新西兰大白兔60只(60眼,均为右眼),行双侧睾丸切除术后随机分为A、B、C、D、E及F 6组(各组10眼)。A组手术后不进行任何处理观察6周;B组手术当日将纤维蛋白胶羊膜棒植入泪道5 s后取出观察6周;C组手术当日植入纤维蛋白胶羊膜棒6周;D组造模(手术2个月后形成干眼模型)后不进行任何处理观察6周;E组造模当日将纤维蛋白胶羊膜棒植入泪道5 s后取出观察6周;F组造模当日植入纤维蛋白胶羊膜棒6周。分别于植入前和植入后2、4及6周行Schirmer I试验(SIT)、角膜荧光素染色(FL)、角膜共聚焦显微镜扫描和泪道黏膜K16的Western blot检查。结果 C组在植入2、4及6周后SIT较植入之前好转,差异有统计学意义(P0.05);C组与A、B组间的SIT在各时间比较,差异有统计学意义(P0.05);在植入2周后,C组FL指标评分较植入之前无明显变化,差异无统计学意义(P0.05);在植入4和6周后C组与A、B组FL指标评分较植入之前轻度好转,差异有统计学意义(P0.05)。在植入2、4及6周后,F组SIT较植入前好转,差异有统计学意义(P0.05),F组FL指标评分较植入之前好转,差异有统计学意义(P0.05);植入后C、F组上皮基底细胞和炎症细胞均减少,差异有统计学意义(P0.05)。免疫荧光染色及Western blot结果显示C、F组泪道黏膜K16阴性表达。结论纤维蛋白胶羊膜棒能够有效地预防和治疗去势雄兔干眼症,延缓干眼症的进展。
[Abstract]:Objective to study the preventive and therapeutic effects of fibrin gel amnion rod on dry eye disease in castrated male rabbits. Methods 60 male New Zealand white rabbits (60 eyes, all right eyes) were randomly divided into A, B, C, D, E and F 6 groups after bilateral orchiectomy (10 eyes in each group were treated with). A without any treatment for 6 weeks). The fibrin glue amniotic rod was implanted into the lacrimal duct for 6 weeks, and the fibrin glue amniotic rod was implanted for 6 weeks on the day of operation in group B, and the fibrin glue amniotic rod was implanted in group C for 6 weeks. The model of group D (dry eye model was formed 2 months after operation) was observed without any treatment for 6 weeks, and the fibrin glue amniotic rod was implanted into lacrimal duct for 6 weeks on the same day of modeling in group E. The rats in group E were taken out for 6 weeks after implantation of fibrin gel into lacrimal duct. Group F was implanted with fibrin gel amnion rod for 6 weeks. Before and 2, 4 and 6 weeks after implantation, Schirmer I test (SIT), fluorescein stained (FL), corneal confocal microscope and lacrimal duct mucosa K16 Western blot were performed. Results after 2 weeks, 4 weeks and 6 weeks after implantation, SIT in group C was better than that before implantation, the difference was statistically significant (P0.05) between); C group and A, B group in each time comparison, the difference was statistically significant (P0.05); 2 weeks after implantation, there was no significant difference in FL score between group C and group C (P0.05). After 4 weeks and 6 weeks after implantation, the FL scores of group C and group A, B were slightly improved compared with those before implantation, the difference was statistically significant (P0.05). After 2 weeks, 4 weeks and 6 weeks after implantation, the SIT in group F was better than that before implantation, the difference was statistically significant (P0.05), the FL score in group), F was better than that before implantation, the difference was statistically significant (P0.05). After implantation C, F group epithelial basal cells and inflammatory cells decreased, the difference was statistically significant (P0.05). Immunofluorescence staining and Western blot showed negative expression of K16 in lacrimal duct mucosa of C and F groups. Conclusion fibrin gel amnion rod can effectively prevent and treat dry eye disease in ovariectomized male rabbits, and delay the progress of dry eye disease in ovariectomized male rabbits.
【作者单位】：南昌大学第一附属医院眼科;南华大学第一附属医院眼科;厦门大学眼科研究所;
【基金】：国家自然科学基金(No:81160118,81400372,81400424,81660158) 江西省远航工程(No:2014022) 江西省自然科学基金重大项目(No:2016ACB21017) 江西省青年科学基金(No:20151BAB215016) 江西省科技支撑计划项目(No:20151BBG70223)
【分类号】：R777.34

【相似文献】