光催化纳米二氧化钛对人鼻咽癌裸鼠模型的治疗作用研究

发布时间：2019-04-01 09:33

【摘要】：目的：研究光催化纳米二氧化钛(TiO_2)对人鼻咽癌裸鼠移植瘤模型的治疗效果，初步探讨其抑癌机理，评估其对机体的影响，为临床研究应用纳米TiO_2光动力疗法治疗鼻咽癌提供理论及实验依据。 方法：建立荷人鼻咽癌裸鼠移植瘤模型，随机分成6组，包括实验组：梯度浓度的纳米TiO_2+紫外光照射组；阴性对照组：单纯纳米TiO_2组与单纯紫外光照射组；空白对照组。其中纳米TiO_2悬液采用瘤内注射，经波长范围330-400nm的紫外光处理后24h，，取各组瘤组织及肝、肾HE染色做病理分析；透射电镜观察肿瘤细胞超微结构的变化及凋亡情况；采用免疫组化检测瘤组织caspase-3的表达，TUNEL法检测细胞凋亡情况，并分析两者相关性。 结果：1、病理学分析，纳米TiO_2光催化治疗后移植瘤组织的局部炎症反应明显（Ⅱ、Ⅲ级），伴随炎症反应的是局部坏死灶形成；而阴性及空白对照组无明显炎症反应（Ⅰ级）。高倍镜观察，各组肝、肾组织均未见明显急性炎症反应或组织损伤表现。 2、透射电镜下观察，实验组可见纳米TiO_2颗粒广泛进入胞浆胞核，肿瘤细胞多有凋亡的形态学改变。 3、实验组caspase-3的阳性表达及TUNEL标记的阳性细胞数显著多于对照组(P0.01)，且阳性表达随TiO_2浓度递增而增强（P0.01）。 结论：1、光催化纳米TiO_2可通过提高移植瘤组织的caspase-3表达活性来诱导细胞凋亡，抑制肿瘤生长，且效果在一定范围内呈TiO_2浓度依赖性。 2、光催化纳米TiO_2通过诱导肿瘤细胞凋亡和介导炎症反应是其发挥抑 癌作用的可能机制。 3、对机体无明显毒副作用，这为光催化纳米TiO_2技术有望应用于临床治疗鼻咽癌提供实验保障。
[Abstract]:Objective: To study the effect of photocatalytic nano-titanium dioxide (TiO _ 2) on the tumor model of human nasopharyngeal carcinoma (NPC) in nude mice. Methods: The model of nude mice with human nasopharyngeal carcinoma (NPC) was established and divided into 6 groups, including the experimental group, the nano-TiO _ 2 + ultraviolet irradiation group, the negative control group, the pure nano-TiO _ 2 group and the pure ultraviolet irradiation group, and the blank control group. The changes of the ultrastructure of the tumor cells and the apoptosis of the tumor cells were observed by transmission electron microscope, and the expression of caspase-3 was detected by immunohistochemistry. The apoptosis of the cells was detected by the TUNEL method, and their correlation was analyzed. Results:1. The pathological analysis showed that the local inflammatory response of the transplanted tumor tissues after the photocatalytic treatment of nano-TiO _ 2 was obviously (鈪

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