熊果酸对糖尿病小鼠视网膜病变的预防作用及其机制探究

发布时间：2019-04-04 19:28

【摘要】：目的:研究熊果酸通过调节胰岛素敏感性、葡萄糖转运、血管新生、炎症反应来预防糖尿病小鼠视网膜病变。方法:选择雄性C57BL/6小鼠作为实验动物,随机分为对照组(N组)、模型组(D组)以及干预组(D+UA组),D组和D+UA组采用STZ腹腔注射的方式建立糖尿病模型,D+UA组给予熊果酸灌胃,测定3组小鼠的胰岛素敏感性、视网膜内糖代谢情况以及视网膜组织中GLUTs、HIF-1α/VEGF/VEGFR2通路和IKKβ/IKBα/NF-kB通路的表达量。结果:D组的AUC显著低于N组,HOMA-IR、视网膜组织含糖量以及GLUT-1、GLUT-3、HIF-1α、VEGF、VEGFR2、IKKβ、IKBα、NF-kB、TNF-α、ICAM-1、VCAM-1、E-selectin的含量显著高于N组;D+UA组的AUC显著高于D组,HOMA-IR、视网膜组织含糖量以及GLUT-1、GLUT-3、HIF-1α、VEGF、VEGFR2、IKKβ、IKBα、NF-kB、TNF-α、ICAM-1、VCAM-1、E-selectin的含量显著低于D组。结论:熊果酸能够增加胰岛素敏感性、降低视网膜内含糖量、抑制视网膜组织中的血管新生过程和炎症反应程度,对糖尿病小鼠视网膜病变具有预防作用。
[Abstract]:Aim: to investigate the role of ursolic acid in preventing diabetic retinopathy in diabetic mice by regulating insulin sensitivity, glucose transport, angiogenesis and inflammation. Methods: male C57BL/6 mice were randomly divided into three groups: control group (N group), model group (D group), D group of (D UA group and D UA group of intervention group to establish diabetic model by intraperitoneal injection of STZ. The insulin sensitivity, glucose metabolism in retina and the expression of GLUTs,HIF-1 伪 / VEGF/VEGFR2 pathway and IKK 尾 / IKB 伪 / NF-kB pathway in retina were measured by intragastric administration of ursolic acid in D UA group. Results: the AUC of group D was significantly lower than that of group N, and the content of glucose and the contents of GLUT-1,GLUT-3,HIF-1 伪, VEGF,VEGFR2,IKK 尾, IKB 伪, NF-kB,TNF- 伪, ICAM-1,VCAM-1,E-selectin in retina of HOMA-IR, group were significantly higher than those in group N (P < 0.05). The AUC in D-UA group was significantly higher than that in D group. The contents of glucose and GLUT-1,GLUT-3,HIF-1 伪, VEGF,VEGFR2,IKK 尾, IKB 伪, NF-kB,TNF- 伪, ICAM-1,VCAM-1,E-selectin in retinal tissue of HOMA-IR, group were significantly lower than those of D group. Conclusion: ursolic acid can increase insulin sensitivity, decrease the content of glucose in the retina, inhibit the angiogenesis and the degree of inflammation in the retinal tissue, and can prevent diabetic retinopathy in mice.
【作者单位】：河北省唐山市丰润区人民医院眼科;
【基金】：河北省自然科学基金项目(C2009001262)~~
【分类号】：R587.2;R774.1

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