经鼻滴入抗白介素5多克隆抗体治疗大鼠变应性鼻炎的机理

发布时间：2019-04-21 10:34

【摘要】：目的：探讨鼻腔滴入抗白介素5多克隆抗体（IL-5抗体）治疗大鼠变应性鼻炎（AR）的机制。方法：采用卵白蛋白（OVA）及氢氧化铝建立大鼠变应性鼻炎（AR）模型，分为模型组（B组），激素组即丙酸氟替卡松组（C组），抗IL-5抗体组（D组），每组6只大鼠。模型建立后C组大鼠鼻腔喷入丙酸氟替卡松，每只每日每侧鼻孔一喷,持续7天；D组大鼠每只每日每侧鼻孔滴入IL-5抗体30L，持续一周；B组大鼠处死；另设阴性对照组（A组）6只，取鼻腔灌洗液及骨髓细胞悬液测定嗜酸性粒细胞百分比，黏膜切片观察鼻黏膜组织病理学改变及GATA-3的表达。结果：D组大鼠鼻腔灌洗液及骨髓腔冲洗液中嗜酸性粒细胞百分比明显低于B组（P 0.01；P 0.01）；病理切片结果显示：B组大鼠的鼻腔黏膜层增厚，并伴有大量炎性细胞浸润，主要以嗜酸细胞为主，且粘膜充血、水肿，纤毛稀疏。而D组大鼠的炎性症状改变明显较B组减轻；免疫组化结果显示B组大鼠鼻腔粘膜组织中GATA-3表达增加，而D组的表达减少，，D组大鼠鼻粘膜免疫组化的平均光密度值明显低于B组（P 0.01），差异有统计学意义。结论： 1.抗IL-5抗体对大鼠变应性鼻炎具有明显治疗作用 2.抗IL-5抗体对大鼠变应性鼻炎的治疗作用在细胞水平上表现为保护大鼠鼻粘膜纤毛结构，降低嗜酸性粒细胞在鼻和骨髓中的浸润或分布。 3.抗IL-5抗体降低嗜酸性粒细胞在鼻黏膜中的浸润与其显著抑制大鼠鼻粘膜GATA-3转录因子的蛋白表达有关。
[Abstract]:Objective: to investigate the mechanism of anti-interleukin-5 polyclonal antibody (IL-5) in the treatment of allergic rhinitis in rats with (AR). Methods: the (AR) model of allergic rhinitis in rats was established by using ovalbumin (OVA) and aluminum hydroxide. They were divided into three groups: model group (group B), corticosteroid group (group C) and anti-IL-5 antibody group (group D) with 6 rats in each group. After the establishment of the model, fluticasone propionate was injected into nasal cavity of rats in group C for 7 days, while rats in group D were injected with IL-5 antibody 30L every day for one week, and rats in group B were killed. The percentage of eosinophils in nasal lavage fluid and bone marrow cell suspension were measured. Histopathological changes of nasal mucosa and the expression of GATA-3 were observed by mucosal sections. Results: the percentage of eosinophils in nasal lavage fluid and bone marrow lavage fluid in group D was significantly lower than that in group B (P 0.01? P 0.01). Pathological sections showed that the nasal mucosa of group B was thickened, accompanied by a large number of inflammatory cell infiltration, mainly eosinophils, mucosal congestion, edema, sparse cilia. The changes of inflammatory symptoms in group D were significantly less than those in group B; The results of immunohistochemistry showed that the expression of GATA-3 in nasal mucosa of group B was increased while that of group D was decreased. The average optical density of nasal mucosa of group D was significantly lower than that of group B (P 0.01). Conclusions: 1. Anti-IL-5 antibody has obvious therapeutic effect on allergic rhinitis in rats. The therapeutic effect of anti-IL-5 antibody on allergic rhinitis in rats was shown to protect the ciliated structure of the nasal mucosa and decrease the infiltration or distribution of eosinophils in the nasal and bone marrow at the cellular level. 3. The decrease of eosinophils infiltration in nasal mucosa by anti-IL-5 antibody is related to its significant inhibition of the protein expression of GATA-3 transcription factor in nasal mucosa of rats.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R765.21

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