维生素E联合川芎嗪对缺血再灌注幼鼠视网膜损伤后的保护作用研究

发布时间：2019-08-24 16:26

【摘要】：目的观察维生素E联合川芎嗪对幼鼠视网膜缺血再灌注及其外周血氧化相关物质的影响,探讨其对损伤后保护的作用机制。方法选取清洁级3周龄幼鼠100只,随机分为5组,每组20只。A组为假造模组、B组为模型组、C组为维生素E干预组、D组为川芎嗪干预组、E组为维生素E联合川芎嗪干预组,再灌注损伤后4 h处死幼鼠,检测外周血清谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-PX)、超氧化物歧化酶(superioxide dismutase,SOD)、活性氧类物质(reactive oxygen species,ROS)、丙二醛(malondialdehyde,MDA),用Tunel染色法检测视网膜细胞凋亡情况。结果幼鼠眼球视网膜细胞Tunel指数B组高于其他4组,E组低于C、D组,D组低于C组(P0.05)。Tunel染色阳性集中在细胞核部位,表现为细胞核固缩。GSH-PX、SOD活性B、C、D、E组均低于A组,C、D、E组高于B组,D、E组高于C组,E组高于D组(P0.05);B、C、D、E组ROS活性和MDA含量均高于A组,C、D、E组均低于B组,且D、E组低于C组,E组低于D组(P0.05)。结论维生素E联合川芎嗪能通过抑制凋亡与抗氧化对缺血再灌注视网膜起到很好的保护作用,两药联合应用效果更佳。
[Abstract]:Objective to observe the effect of vitamin E combined with ligustrazin on retinal ischemia-reperfusion and its peripheral blood oxidation-related substances in young rats, and to explore the protective mechanism of vitamin E combined with ligustrazine on retinal ischemia-reperfusion and peripheral blood oxidation-related substances in young rats. Methods 100 clean grade 3-week-old young rats were randomly divided into 5 groups with 20 rats in each group. Group A was false model group, group B was model group, group C was vitamin E intervention group, group D was ligustrazin intervention group, group E was vitamin E combined with ligustrazine intervention group, the young rats were killed 4 hours after reperfusion injury, and peripheral serum glutathione peroxidase,GSH-PX and superioxide dismutase,SOD were measured. The apoptosis of retinal cells was detected by Tunel staining with reactive oxygen species (reactive oxygen species,ROS) and malondialdehyde (malondialdehyde,MDA). Results the Tunel index of retinal cells in group B was higher than that in the other four groups, the Tunel index in group E was lower than that in group C, and the positive staining in group D was lower than that in group C (P 0.05). The activity of GSH-PX,SOD in group C, group D, group E was lower than that in group A, group C, group D, group E was higher than group B, group E was higher than group C, and group E was higher than group D (P 0.05). B, C, D, E group ROS activity and MDA content were higher than group A, C, D, E group were lower than group B, D, E group was lower than C group, E group was lower than D group (P 0.05). Conclusion Vitamin E combined with ligustrazin can play a good protective role in ischemia-reperfusion retinal by inhibiting apoptosis and antioxidation, and the combined application of the two drugs is more effective.
【作者单位】：中国医科大学附属第一医院新生儿科;
【分类号】：R774.1

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