聚合酶链反应法快速诊断肝硬化患者腹水细菌感染临床应用研究

发布时间：2017-12-30 22:35

  本文关键词：聚合酶链反应法快速诊断肝硬化患者腹水细菌感染临床应用研究　出处：《中华医院感染学杂志》2016年19期 　论文类型：期刊论文

  更多相关文章： 聚合酶链反应 肝硬化 自发性腹膜炎 SrRNA

【摘要】：目的应用聚合酶链反应(PCR)检测细菌16SrRNA基因,探讨其在辅助诊断肝硬化自发性腹膜炎(SBP)中的临床应用价值。方法选取2014年12月-2015年10月医院感染病科77例肝硬化失代偿期合并腹水患者进行研究,以细菌16SrRNA基因为靶序列,在保守区和特异区分别选取特异性序列合成引物,建立快速诊断方法,检测临床肝硬化失代偿期患者腹水中细菌DNA,并将其结果与传统方法进行比较,数据采用SPSS16.0统计软件进行处理。结果 77份临床样本中PCR法16SrRNA通用引物阳性扩增50例,阳性率64.9%,特异引物阳性扩增15例,阳性率19.5%;以腹水细菌培养阳性或(和)腹水PMN≥250×106/L或(和)腹膜刺激征阳性且正规抗感染治疗有效者作为诊断SBP金标准,此时PCR法灵敏度为100.0%,特异度为54.0%,阳性预测值为54.0%,阴性预测值为100.0%,二者差异有统计学意义(χ~2=22.453,P0.01)。结论与传统方法相比,PCR法是一种更敏感且简便快速的方法,在肝硬化失代偿期患者腹水细菌检测中具有一定的临床应用价值。
[Abstract]:Objective to detect 16s rRNA gene by polymerase chain reaction (PCR). Study on the role of SBP in the Auxiliary diagnosis of spontaneous peritonitis in cirrhotic patients. Methods from December 2014 to October 2015, 77 patients with ascites in decompensated stage of liver cirrhosis were studied. Using 16s rRNA as the target sequence, primers were synthesized in the conserved region and the specific region, and a rapid diagnostic method was established to detect bacterial DNA in ascites of patients with decompensated cirrhosis. The results were compared with the traditional method and the data were processed by SPSS16.0 software. Results 50 cases of PCR 16s rRNA universal primer positive amplification were performed in 77 clinical samples. The positive rate was 64.9% and the positive rate of specific primers was 19.5% in 15 cases. Patients with positive bacterial culture or / and ascites PMN 鈮，

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