结肠慢传输型便秘大鼠肠道菌群的变化及益生菌的干预效果

发布时间：2018-01-19 04:38

本文关键词： 慢传输型便秘肠道菌群益生菌　出处：《广东医学》2016年03期 　论文类型：期刊论文

【摘要】：目的探讨结肠慢传输型便秘(STC)大鼠肠道菌群变化及益生菌的干预效果。方法采用复方地芬诺酯灌胃的方法建立大鼠STC模型成功后,将32只STC大鼠随机分为STC组(n=16)、STC干预组(n=16),另设正常SD大鼠(n=16)为对照组。利用16S rRNA Real-time PCR技术测定肠道菌群,消化道排空时间采用活性碳灌胃法测定。首先对STC组大鼠粪便肠道菌群进行检测,再根据肠道菌群检测结果对STC大鼠每天灌胃乳酸杆菌及双歧杆菌各0.5 m L,观察干预后实验大鼠粪便主要功能细菌及消化道排空时间变化。结果 STC组和对照组粪便乳酸杆菌、双歧杆菌数分别为4.22±0.07、5.26±0.10和6.51±0.10、7.68±0.17,STC组低于对照组,组间比较差异有统计学意义(P0.01),粪便总细菌、厚壁菌门、拟杆菌门、梭菌群Ⅳ等主要肠道优势菌群数两组间比较差异无统计学意义(P0.05)。经乳酸杆菌、双歧杆菌干预后粪便总细菌、厚壁菌门、拟杆菌门、梭菌群Ⅳ3组间比较差异无统计学意义(P0.05),STC组、STC干预组、对照组乳酸杆菌、双歧杆菌数分别为4.20±0.07、5.21±0.10和6.47±0.09、6.47±0.05和6.50±0.08、7.79±0.06,STC组明显低于STC干预组和对照组,组间比较差异有统计学意义(P0.01),干预后STC、STC干预组及对照组粪便活性碳排出时间分别为(5.56±0.50)d、(2.55±0.67)d、(0.77±0.01)d,STC组慢于其余两组,组间比较差异有统计学意义(P0.01)。结论结肠STC大鼠粪便中肠道乳酸杆菌及双歧杆菌数明显减少,主要肠道优势菌群数未发生改变,经乳酸杆菌及双歧杆菌干预后可恢复结肠STC大鼠肠道菌群状态及消化肠道排空时间。
[Abstract]:Objective to investigate the changes of intestinal flora in rats with slow transit constipation of colon and the intervention effect of probiotics. Thirty-two STC rats were randomly divided into STC group (n = 16) and STC-treated group (n = 16). The intestinal flora was determined by 16s rRNA Real-time PCR technique. The emptying time of digestive tract was measured by active carbon perfusion. First, the fecal microflora of rats in STC group was detected. According to the results of intestinal microflora test, STC rats were given intragastric administration of Lactobacillus and Bifidobacterium 0.5 mL each day. Results the fecal Lactobacillus and Bifidobacterium numbers in STC group and control group were 4.22 卤0.07 respectively. 5.26 卤0.10 and 6.51 卤0.107.68 卤0.17 of STC group were lower than that of the control group, the difference between the two groups was statistically significant (P 0.01), total bacteria in feces. There was no significant difference in the number of dominant intestinal bacteria between the two groups. After the intervention of Lactobacillus Bifidobacterium and Bifidobacterium the total bacteria in feces and the phylum thuringiensis. There was no significant difference between the two groups. The number of Lactobacillus and Bifidobacterium in the control group was 4.20 卤0.07 respectively. 5.21 卤0.10 and 6.47 卤0.09 卤6.47 卤0. 05 and 6. 50 卤0. 08 卤7. 79 卤0. 06 of STC group were significantly lower than those of STC intervention group and control group. The difference between the two groups was statistically significant (P 0.01). The excretion time of fecal active carbon in STC intervention group and control group was 5.56 卤0.50 days after intervention. The STC group (2.55 卤0.67) was slower than the other two groups in the STC group (0.77 卤0.01). Conclusion the number of Lactobacillus and Bifidobacterium in feces of colonic STC rats was significantly decreased, but the number of dominant bacteria in the main intestinal tract did not change. After the intervention of Lactobacillus and Bifidobacterium, the intestinal flora and digestive tract emptying time of colonic STC rats were restored.
【作者单位】：中山大学附属第六医院肛肠外科;
【基金】：广东省自然科学基金资助项目(编号:s2013010013587) 广东省科技计划项目(编号:2014A020212123)
【分类号】：R574.62
【正文快照】： 结肠慢传输型便秘(slow transit constipation,STC)是功能性便秘最重要的一种类型,主要表现为大便干结、大便次数减少、排便间隔时间延长[1],其发病原因及机制尚不清楚,临床治疗效果还不令人满意[2]。新近临床研究发现,肠道菌群异常可能在功能性便秘的发生、发展方面起着重要

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