SMVT大鼠肠道组织中HIF-1α、VEGF表达变化及意义

发布时间：2018-01-30 14:26

本文关键词： 肠系膜上静脉血栓形成低氧诱导因子α 血管内皮生长因子侧支循环大鼠　出处：《山东医药》2016年41期 　论文类型：期刊论文

【摘要】：目的观察肠系膜上静脉血栓形成(SMVT)大鼠肠道组织中HIF-1α、VEGF的表达变化并探讨其意义。方法将64只雄性SD大鼠随机分为实验组和假手术组,每组32只。实验组结扎法建立SMVT单纯性周围型模型,建模后8、24、48、72 h分批处死大鼠(每次8只);假手术组仅打开腹腔,不阻断血运,术后8、24、48、72 h分批处死大鼠(每次8只)。观察肠道大体变化;HE染色观察大鼠肠道组织形态学变化;RT-q PCR法、免疫组化染色及Western blotting法检测大鼠肠道组织中HIF-1α、VEGF mRNA及蛋白表达。结果大体观察及HE染色结果示,实验组大鼠均出现不同程度的血液淤滞,且随时间延长淤血程度逐渐减轻;假手术组大鼠肠道组织均无明显病理变化。RT-q PCR、免疫组化染色及Western blotting结果示,实验组较假手术组对应各时间点HIF-1α、VEGF mRNA及蛋白表达明显升高(P均0.05)。结论 SMVT大鼠肠道组织中HIF-1α、VEGF表达升高,HIF-1-VEGF信号通路可能促进SMVT中侧支循环的建立。
[Abstract]:Objective to observe HIF-1 伪 in intestinal tissue of rats with superior mesenteric vein thrombosis. Methods 64 male Sprague-Dawley rats were randomly divided into experimental group and sham-operated group with 32 rats in each group. The experimental group was ligated to establish a simple peripheral SMVT model. The rats were killed in batches (8 rats each time) at 48 hours after modeling. In the sham-operation group, the abdominal cavity was opened and the blood flow was not blocked. The rats were killed in groups (8 rats each time) at 824 hours and 48 minutes after operation. The gross changes of the intestine were observed. The changes of intestinal tissue morphology were observed by HE staining. RT-q PCR method, immunohistochemical staining and Western blotting method were used to detect HIF-1 伪 in rat intestinal tissue. Results the results of gross observation and HE staining showed that the rats in the experimental group had different degrees of blood stasis, and the degree of congestion gradually decreased with the prolongation of time. There were no obvious pathological changes in intestinal tissue of sham-operated rats. RT-q PCR, immunohistochemical staining and Western blotting showed. Compared with the sham operation group, the expression of HIF-1 伪 -VEGF mRNA and protein in the experimental group was significantly higher than that in the sham operation group at each time point (P 0.05). Conclusion HIF-1 伪 in intestinal tissue of SMVT rats was significantly increased. The increased expression of VEGF may promote the establishment of collateral circulation in SMVT.
【作者单位】：华北理工大学附属医院;
【基金】：河北省卫生厅医学科学研究重点课题(20100130)
【分类号】：R572.3
【正文快照】： 肠系膜上静脉血栓形成(SMVT)是较少见的血管源性急腹症。研究显示,SMVT发病占肠缺血性疾病的5%~10%[1]。低氧诱导因子1(HIF-1)是一种在低氧环境下介导适应反应的关键性转录调控因子,由HIF-1α与HIF-1β亚基组成的异源二聚体[2]。HIF-1α是决定其活性的主要氧调节亚基,在多种病

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