腹水COX-2 mRNA的表达及意义

发布时间：2018-02-16 05:59

本文关键词： 腹水 COX- mRNA 荧光定量PCR 肿瘤　出处：《中山大学学报(医学科学版)》2016年02期 　论文类型：期刊论文

【摘要】：【目的】通过检测腹水COX-2 m RNA的表达水平,探讨COX-2 m RNA对腹水鉴别诊断的价值和意义。【方法】收集2014年6月至2015年2月期间到我院就诊并明确诊断的恶性腹水34例,良性腹水15例。采用实时荧光定量PCR方法检测腹水中COX-2 m RNA的表达水平,比较COX-2 m RNA在良恶性腹水中表达的差异,及在不同原发疾病中的表达情况。【结果】34例恶性腹水及15例良性腹水均扩增出COX-2,良恶性腹水中COX-2 m RNA的相对表达水平与性别、年龄构成比无关系,差异无统计学意义(P0.05)。恶性腹水和良性腹水COX-2 m RNA的表达水平分别为7.93(5.04,16.58)和0.95(0.57,1.79),即恶性腹水COX-2 m RNA的表达水平显著高于良性腹水,差异有统计学意义(P0.05)。恶性腹水COX-2m RNA的表达水平在不同原发疾病中差异无统计学意义(P0.05),良性腹水COX-2 m RNA表达水平在肝硬化和结核性腹膜炎中分别为1.55(0.55,2.55)和0.87(0.61,1.38),差异无统计学意义(P0.05)。【结论】COX-2 m RNA在恶性腹水中的表达水平显著高于良性腹水,在鉴别诊断腹水性质中具有重要的参考价值。
[Abstract]:[objective] to investigate the value and significance of COX-2 m RNA in differential diagnosis of ascites by detecting the expression of COX-2 m RNA in ascites. [methods] 34 cases of malignant ascites were collected from June 2014 to February 2015. The expression level of COX-2 m RNA in ascites was detected by real-time fluorescence quantitative PCR, and the difference of COX-2 m RNA expression in benign and malignant ascites was compared. [results] COX-2 was amplified from 34 cases of malignant ascites and 15 cases of benign ascites. The relative expression level of COX-2 m RNA in benign and malignant ascites was not related to sex and age ratio. The expression levels of COX-2 m RNA in malignant ascites and benign ascites were 7.93 ~ 5.04 ~ 16.58) and 0.95 ~ 0.57 ~ 1.79 respectively. The expression level of COX-2 m RNA in malignant ascites was significantly higher than that in benign ascites, and the expression of COX-2 m in malignant ascites was significantly higher than that in benign ascites, and the expression of COX-2 m in malignant ascites was significantly higher than that in benign ascites. There was no significant difference in the expression level of COX-2m RNA in malignant ascites in different primary diseases. The expression level of COX-2m RNA in benign ascites was 1.55 / 0.55 / 2.55 in cirrhosis and 0.87 / 0.61 / 1.38 in tuberculous peritonitis, respectively. There was no significant difference between the expression levels of COX-2m RNA in benign ascites and patients with liver cirrhosis and tuberculous peritonitis (P < 0.05), and there was no significant difference in the expression of COX-2m RNA between benign ascites and tuberculous peritonitis. [conclusion] the expression of COX-2 m RNA in malignant ascites is significantly higher than that in benign ascites. It has important reference value in the differential diagnosis of ascites.
【作者单位】：中山大学附属第五医院消化内科;东莞东华医院消化内科;东莞东华医院肾内科;
【基金】：广东省科技计划项目(2014A020212387) 珠海市科技计划项目(2014D0401990005)
【分类号】：R57

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