低氧对苯肾上腺素诱导的豚鼠肠系膜动脉收缩反应的影响

发布时间：2018-02-24 05:42

本文关键词： 低氧肠系膜动脉压力肌动图技术豚鼠　出处：《实用医学杂志》2015年06期 　论文类型：期刊论文

【摘要】：目的:探讨不同时段低氧对苯肾上腺素(phenylephrine,PE)诱导的豚鼠肠系膜动脉收缩反应的影响及其作用机制。方法:应用压力肌动图技术,在急性分离的豚鼠肠系膜动脉2级血管(直径300~400μm)上,观察20、40和60 min低氧(95%CO2和5%O2混合)对内皮非依赖的血管收缩剂PE收缩血管直径的影响。结果:PE(0.1~100μmol/L)可以浓度依赖性收缩豚鼠肠系膜动脉。低氧引起生理盐溶液p H值从7.4下降到6.3。低氧能显著抑制PE引起的血管收缩幅度,用正常生理盐溶液洗脱20 min此抑制作用仍难以恢复。低氧抑制PE引起的肠系膜动脉的收缩反应具有时间依赖性,抑制程度依次为低氧60 min40min20 min。将生理盐溶液p H降低至6.3也可抑制PE对血管收缩的作用。结论 :低氧能够时间依赖性地抑制PE对豚鼠肠系膜动脉的收缩作用,其作用机制可能与p H的下降有关。
[Abstract]:Objective: to investigate the effects of hypoxia on phenylephrinephrinepe-induced contraction of mesenteric artery in guinea pigs at different periods of time and its mechanism. On acute isolated mesenteric artery grade 2 (300 渭 m in diameter) of guinea pig mesenteric artery, The effects of 20, 40 and 60 min hypoxia on the diameter of endothelium-independent vasoconstrictor PE constrictor PE were observed. Results the concentration-dependent contraction of guinea pig mesenteric artery was induced by hypoxia. The pH value of physiological salt solution induced by hypoxia was determined from 1. 1 渭 mol / L to 0. 1 渭 mol / L). 7.4 decreased to 6.3. Hypoxia could significantly inhibit the amplitude of vasoconstriction induced by PE. The inhibition was still difficult to recover when eluted with normal physiological salt solution for 20 min. Hypoxia inhibited the contraction of mesenteric artery induced by PE in a time-dependent manner. The degree of inhibition was 60 min40min20 after hypoxia. Lowering pH to 6.3 in physiological salt solution could also inhibit the vasoconstriction of PE. Conclusion: hypoxia can inhibit the contraction of mesenteric artery in guinea pigs in a time-dependent manner. The mechanism may be related to the decrease of pH.
【作者单位】：石河子大学医学院生理学教研室;新疆军区乌鲁木齐第六干休所;石河子大学医学院病理生理学教研室;
【基金】：国家自然科学基金资助项目(编号:3126047,3460264,81460098)
【分类号】：R572.3

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