B7-H4对ConA诱导小鼠肝损伤保护作用的实验研究

发布时间：2018-02-24 05:23

本文关键词： B-H Con A-诱导性肝损伤保护作用机制　出处：《四川大学学报(医学版)》2015年06期 　论文类型：期刊论文

【摘要】：目的探讨B7-H4对免疫性损伤肝脏的保护作用及其机制。方法将KM小鼠分为4组(每组15只):生理盐水组(A组)、pcDNA3.1-mB7-H4-Fc组(B组)、pcDNA3.1组(C组)和刀豆球蛋白A(Con A)组(D组)。B组、C组和D组动物均通过尾静脉注射Con A 25mg/kg制备肝损伤模型。在Con A注射前1d,分别给予B组每只小鼠注射pcDNA3.1-mB7-H4-Fc 100μg和C组每只小鼠注射pcDNA3.1 100μg。并于注射Con A后12h、24h和48h,各组取5只小鼠,摘眼球取血,分离血清行白介素-4(IL-4)、γ-干扰素(IFN-γ)、谷丙转氨酶(ALT)和谷草转氨酶(AST)浓度的测定;引颈处死小鼠后取肝脏,用于组织病理学检测。结果小鼠血清IL-4、IFN-γ、ALT和AST水平在注射Con A后各时点,B、C和D三组较A组均有升高(P0.05),但B组较C组和D组减低,B组与C组间差异有统计学意义(P0.01)。小鼠肝组织切片经HE染色,光学显微镜下可见,B组小鼠肝脏损伤均比C组和D组有不同程度的减轻。结论初步证实了B7-H4对Con A诱导的肝脏免疫性损伤具有保护作用。B7-H4可能是通过抑制IL-4和IFN-γ的产生和/或分泌而发挥对肝脏保护的作用。
[Abstract]:Objective to investigate the protective effect of B7-H4 on immune liver injury and its mechanism. Methods km mice were divided into 4 groups (15 rats in each group: pcDNA3.1-mB7-H4-Fc group, pcDNA3.1-mB7-H4-Fc group) and concanavalin A concanavalin A (Con A) group. Rats in group D and group D were injected with Con A 25 mg / kg via tail vein to make liver injury model. Each mouse in group B and group C were injected with pcDNA3.1-mB7-H4-Fc 100 渭 g and pcDNA3.1 100 渭 g 1 day before injection of Con A. after 12 h and 48 h of Con A injection, 5 mice in each group were taken from each group. The serum levels of IL-4, IFN- 纬, alanine aminotransferase (alt) and aspartate aminotransferase (AST) were determined, and the liver was taken after the mice were killed by neck drawing. Results the levels of serum IL-4 IFN- 纬 -alt and AST in mice were higher than those in group A at each time point after Con A injection, but the difference between group B and group C was significantly lower than that in group C and group D. The sections of rat liver were stained with HE. Under optical microscope, the liver injury in group B was significantly less than that in group C and group D. ConclusionThe protective effect of B7-H4 on liver immune injury induced by Con A. B7-H4 may be mediated by inhibiting IL-4 and IFN- 纬. And / or secreted to protect the liver.
【作者单位】：海南医学院附属医院核医学科;广东省医学分子诊断重点实验室;
【基金】：海南省卫生厅科研基金项目(琼卫2010-3)资助
【分类号】：R575

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