红景天提取物鸟嘌呤对大鼠肝纤维化治疗作用的观察

发布时间：2018-02-26 17:21

本文关键词： 红景天鸟嘌呤大鼠四氯化碳肝纤维化　出处：《青海大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:研究并探讨红景天提取物鸟嘌呤对实验性大鼠肝纤维化的治疗作用。方法:健康雄性SD大鼠70只,随机选取16只作为空白对照组,其余54只大鼠用作肝纤维化动物模型的建立,置于相同环境。用CCl4灌胃给药,剂量为1.0ml/Kg·BW,构建肝纤维化模型,空白对照组用生理盐水灌胃,剂量为1.0ml/Kg·BW,给药时间为每天相对固定的时间,一天一次。连续60天灌胃后,分别取空白对照组、建立肝纤维化模型组各7只动物,禁食不禁水过夜,以0.5%水合氯醛麻醉后,腹腔动脉取血,分离血清后进行肝纤维化指标的检测。经过常规病理学检查之后,依据表2.2判断模型是否成功。验证造模成功后,将建立肝纤维化模型组随机分为模型组A:生理盐水组13只,模型组B:鸟嘌呤低剂量组13只,模型组C:鸟嘌呤高剂量组12只,A、B、C三组分别给予生理盐水1.0ml/Kg·BW,低剂量鸟嘌呤0.056 g/Kg·BW,高剂量鸟嘌呤0.112 g/Kg·BW灌胃给药,空白对照组同样取剩余的7只动物给予生理盐水1.0 ml/Kg·BW灌胃。连续60天后,各组动物禁食不禁水过夜,第二天以0.5%水合氯醛皮下注射麻醉后取腹腔动脉血,分离血清后进行肝纤维化指标的检测。用10%福尔马林溶液固定肝脏后,对其进行免疫组化检查。通过血清指标检测及病理学观察,判断药物疗效。结果:60天造模结束时11只大鼠死亡,其中包括空白对照组2只,建立肝纤维化模型组9只,死亡原因考虑因灌胃操作不当及四氯化碳中毒而死亡。造模组大鼠常规病理分期结果:4期5只、3期2只,生化指标示:模型组与空白对照组相比,ALT、AST有统计差异(P0.05),LN、CIV无统计差异。结合病理及生化结果示:造模成功。各组给予药物治疗60天后共出现9只大鼠死亡:空白对照组1只,模型组A:4只、模型组B:3只、模型组C:1只,死亡原因多考虑因灌胃给药操作不当死亡。生化指标显示:ALT、CIV、LN有统计学差异,(p0.05)。AST无统计学意义。免疫组化结果示:鸟嘌呤对肝纤维化有治疗作用,且高剂量较低剂量效果显著。结论:1.鸟嘌呤灌胃后肝脏相关生化指标较生理盐水组显著降低,提示鸟嘌呤对肝脏具有保护作用,能改善四氯化碳诱导引起的肝纤维化大鼠的肝功能;2.高剂量的鸟嘌呤对改善四氯化碳引起的大鼠肝纤维化的程度明显高于低剂量组。
[Abstract]:Objective: to study the therapeutic effect of guanine extract from Rhodiola on experimental hepatic fibrosis in rats. Methods: 70 healthy male SD rats were randomly selected as the blank control group. The other 54 rats were used as animal models of liver fibrosis and placed in the same environment. The model was established by intragastric administration of CCl4 at a dose of 1.0 ml / kg 路BW, while the blank control group was given normal saline. The dose was 1.0 ml / kg 路BW, the administration time was relatively fixed daily, once a day. After 60 days of continuous gastric perfusion, the rats in the control group were taken from the blank control group. The rats in the liver fibrosis model group (n = 7) were fasted for the night. After anesthesia with 0.5% chloral hydrate, the rats were anesthetized with 0.5% chloral hydrate. Blood was taken from celiac artery, serum was separated and liver fibrosis index was detected. After routine pathological examination, the model was judged according to Table 2.2. The liver fibrosis model group was randomly divided into model group A: normal saline group (n = 13), model group B: guanine low dose group (n = 13), model group B: guanine low dose group (n = 13). Model group C: 12 rats in high dose guanine group were given normal saline 1.0 ml / kg 路BW, low dose guanine 0.056 g / kg 路BW, high dose guanine 0.112 g / kg 路BW respectively. In the blank control group, the remaining 7 animals were also given normal saline 1.0 ml/Kg 路BW intragastrically. After 60 days of fasting, the animals in each group fasted and stayed overnight, and the abdominal arterial blood was taken after subcutaneous injection of 0.5% chloral hydrate in the next day. After separating serum, the liver fibrosis index was detected. After the liver was fixed with 10% formalin solution, the liver was examined by immunohistochemistry. Results at the end of 60 days, 11 rats died, including 2 rats in the blank control group and 9 rats in the model group of liver fibrosis. The cause of death was due to improper gavage and carbon tetrachloride poisoning. The biochemical indexes showed that there was no statistical difference between the model group and the blank control group in ALTN AST. Combined with pathological and biochemical results, the model was successfully established. After 60 days of drug treatment, 9 rats died in each group: 1 in the blank control group. Model group A: 4, model group B: 3, model group C: 1, The cause of death was due to improper oral administration. Biochemical indexes showed that there was no significant difference between the two groups. Immunohistochemical results showed that guanine had a therapeutic effect on hepatic fibrosis. Conclusion: 1. The relative biochemical indexes of liver after guanine administration were significantly lower than those in saline group, suggesting that guanine has protective effect on liver. It can improve the liver function of rats induced by carbon tetrachloride. The degree of improving hepatic fibrosis induced by carbon tetrachloride in rats with high dose guanine is significantly higher than that in the low dose group.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R575.2

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