微小RNA-7敲减对ConA诱导的小鼠急性肝损伤的影响

发布时间：2018-02-28 16:30

本文关键词： 微小RNA- 急性肝损伤 CD~+T细胞　出处：《中国病理生理杂志》2017年09期 　论文类型：期刊论文

【摘要】：目的:研究微小RNA-7(miR-7)敲减(KD)对急性肝损伤(ALI)模型小鼠的影响。方法:野生型(WT)小鼠和miR-7KD小鼠腹腔注射30 mg/kg刀豆蛋白(ConA)建立急性肝损伤模型;48 h后,观察小鼠肝脏的形态、重量及其脏器指数变化;HE染色观察小鼠肝脏组织病理学变化;血清学方法检查血清中谷丙转氨酶(ALT)的水平;ELISA法检测血清中细胞因子IL-4和IFN-γ的水平;流式细胞术检测肝脏组织中CD4~+T细胞的比例及其相关的细胞因子IL-4和IFN-γ的表达变化。结果:与对照组相比,miR-7敲减后急性肝损伤小鼠的肝脏组织颜色变浅,重量减轻,重量指数明显增加(P0.05);HE染色显示miR-7KD小鼠血清炎症细胞浸润显著增多;血清学方法检测发现急性肝损伤小鼠血清ALT的水平明显上升(P0.05);ELISA法检测显示miR-7KD小鼠血清中IFN-γ水平明显升高(P0.01),而IL-4的表达水平则明显降低(P0.01);流式细胞术检测结果显示,miR-7KD小鼠肝脏中CD4+T细胞比例显著升高(P0.01),其相关的细胞因子IFN-γ的表达水平也显著上调(P0.01),而IL-4的水平没有发生明显变化。结论:敲减miR-7基因可明显促进ConA诱导的小鼠急性肝损伤。
[Abstract]:Objective: to study the effect of minimal RNA-7miR-7 knockdown (KDD) on acute liver injury (ALI) mice. Methods: the acute liver injury model was established by intraperitoneal injection of concanavalin Cona for 30 mg/kg in wild-type mice and miR-7KD mice, and the morphology of liver was observed. The changes of weight and organ index were observed by HE staining, the levels of serum alanine aminotransferase (alt) and the levels of cytokines IL-4 and IFN- 纬 in serum were detected by serological method and Elisa respectively. The percentage of CD4T cells and the expression of related cytokines IL-4 and IFN- 纬 in liver tissue were detected by flow cytometry. Results: compared with the control group, the color and weight of liver tissue of mice with acute liver injury were lighter and lighter than those of control group. The weight index increased significantly (P 0.05) and HE staining showed that the infiltration of inflammatory cells in serum of miR-7KD mice was significantly increased. Serum levels of ALT in mice with acute liver injury were significantly increased by serological method. Elisa showed that the level of IFN- 纬 in serum of miR-7KD mice was significantly higher than that of P0.01G, while the expression of IL-4 was significantly decreased in mice with acute liver injury, and the results of flow cytometry showed that the level of IFN- 纬 in serum of miR-7KD mice was significantly higher than that of P0.01G, and that the expression of IL-4 was significantly decreased by flow cytometry. The proportion of CD4 T cells in the liver of miR-7KD mice was significantly increased, and the expression of IFN- 纬, the related cytokine, was also up-regulated, but the level of IL-4 did not change significantly. Conclusion: knockout of miR-7 gene can significantly promote the acute liver injury induced by ConA in mice.
【作者单位】：遵义医学院免疫学教研室暨贵州省生物治疗人才基地;遵义医学院贵州省普通高等学校特色药物肿瘤防治特色重点实验室;
【基金】：国家自然科学基金资助项目(No.31370918) 贵州省高层次创新人才计划(黔科合人才(2016)4031号) 遵义医学院优秀青年人才计划项目(No.15ZY-001)
【分类号】：R575

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