重症急性胰腺炎大鼠肠黏膜屏障及肠道菌群的变化

发布时间：2018-02-28 18:33

本文关键词： 重症急性胰腺炎肠黏膜屏障肠道微生态实时荧光定量-聚合酶链式反应　出处：《生物医学工程学杂志》2015年02期 　论文类型：期刊论文

【摘要】：本文旨在探讨重症急性胰腺炎(SAP)大鼠肠黏膜屏障、肠道菌群的变化和细菌移位的情况。将健康雄性SD大鼠随机分为对照组(n=10)和实验组(n=14),采用5%牛磺胆酸钠胰胆管逆行注射法制作重症急性胰腺炎模型,24h后处死,测定血清淀粉酶及血浆内毒素水平,观察胰腺、小肠病理改变并评分,检测腹腔脏器细菌移位率,实时荧光定量聚合酶链式反应法(RT-PCR)定量分析肠道内菌群数量。结果显示:实验组大鼠肠腔内大肠埃希菌属计数明显升高(9.72±3.58,P0.01),乳酸杆菌属(0.67±0.34,P0.01)和双歧杆菌属(4.59±3.42,P0.05)计数明显减少,血浆内毒素测定阳性率增高(P0.01),腹腔脏器细菌移位率升高(P0.01或P0.05),胰腺及小肠出现明显病理损伤。表明重症急性胰腺炎大鼠肠黏膜屏障受损,出现肠道菌群失调、细菌及内毒素移位,其与多脏器感染的发生、发展或有关联。
[Abstract]:The purpose of this study was to investigate the intestinal mucosal barrier in rats with severe acute pancreatitis (SAP). The changes of intestinal flora and bacterial translocation. Healthy male SD rats were randomly divided into two groups: control group (n = 10) and experimental group (n = 14). The model of severe acute pancreatitis was made by retrograde injection of sodium taurocholate into pancreaticobiliary duct for 24 hours. The levels of serum amylase and plasma endotoxin were measured, the pathological changes of pancreas and small intestine were observed, and the bacterial translocation rate of abdominal viscera was detected. Real-time quantitative polymerase chain reaction (RT-PCR) method was used to determine the number of intestinal bacteria in rats. The results showed that the number of Escherichia coli, Lactobacillus, Lactobacillus, Bifidobacterium and Bifidobacterium were significantly increased by 9.72 卤3.58, 0.67 卤0.34 and 4.59 卤3.42P0.05, respectively. The positive rate of plasma endotoxin test was higher than that of P0.01A, the bacterial translocation rate of abdominal viscera increased P0.01 or P0.05A, and the pancreas and small intestine showed obvious pathological damage, which indicated that the intestinal mucosal barrier was damaged and intestinal flora was disordered in rats with severe acute pancreatitis. Bacterial and endotoxin translocation, which is associated with the occurrence, development or association of multiple organ infections.
【作者单位】：新疆医科大学第一附属医院消化科;四川大学华西医院消化科;
【基金】：新疆维吾尔自治区自然科学基金资助项目(201233146-15)
【分类号】：R576

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