IgG免疫复合物致小鼠肠道黏膜损伤机制的初步试验研究

发布时间：2018-03-02 08:26

本文关键词： 溃疡性结肠炎结肠黏膜损伤肥大细胞免疫球蛋白G　出处：《中国免疫学杂志》2017年08期 　论文类型：期刊论文

【摘要】：目的:探讨IgG免疫复合物对小鼠肠黏膜损伤的局部组织病理特点,以及相应的细胞与分子机制,为临床上食物不耐受诱发溃疡性结肠炎的致病机制及分子诊断提供一定依据。方法:选用6周龄的BALB/c雌鼠作为实验对象进行造模,分为对照组(A组)、兔肠黏膜蛋白免疫组(B组)、DSS诱导组(C组)、兔肠黏膜蛋白免疫合并DSS诱导组(D组)共4组。造模成功后收集血清与结肠组织进行相关检测。结果:B组小鼠血清及结肠黏膜中IgG水平上升,肠黏膜组织中少量肥大细胞活化,以及炎性细胞浸润;C组小鼠表现为典型急性溃疡性结肠炎表征,黏膜和黏膜固有层大量炎症细胞浸润,肠黏膜组织中炎症因子水平上升,黏膜上皮紧密连接减弱;D组小鼠血清及结肠黏膜中IgG水平显著升高,组织学表现与DSS诱导组相似,并有显著肥大细胞浸润与活化。结论:IgG免疫复合物通过介导肥大细胞活化脱颗粒,诱发肠黏膜上皮损伤,可能与肥大细胞释放的促炎因子导致的局部炎症以及对上皮细胞紧密连接蛋白的表达影响有关。
[Abstract]:Objective: to investigate the local histopathological features and the corresponding cellular and molecular mechanisms of intestinal mucosal injury induced by IgG immune complex in mice. To provide some basis for the pathogenesis and molecular diagnosis of ulcerative colitis induced by food intolerance. Methods: BALB/c female mice aged 6 weeks were selected as the experimental objects to model the ulcerative colitis. The rats were divided into 4 groups: control group (group A), rabbit intestinal mucosal protein immunization group (group B) and rabbit intestinal mucosal protein immunization with DSS induction group (group C). The level of IgG in serum and colonic mucosa of mice in group B was increased. In group C, a small amount of mast cells were activated in intestinal mucosa and inflammatory cells infiltrated. The mice in group C showed typical acute ulcerative colitis. A large number of inflammatory cells were infiltrated in mucosa and lamina propria mucosa, and the level of inflammatory factors in intestinal mucosa was increased. The level of IgG in serum and colonic mucosa of group D was significantly higher than that of group D, and the histological findings were similar to those of DSS induced group. Conclusion the weight IgG immune complex induces intestinal mucosal epithelium injury by mediating mast cell activation and degranulation. It may be related to the local inflammation caused by proinflammatory factor released by mast cells and the effect on the expression of tight junction protein in epithelial cells.
【作者单位】：广州医科大学附属第二医院广东省过敏反应与免疫重点实验室;广州医科大学附属第二医院消化内科;
【基金】：广东省省级科技计划项目(No.2013B022000095) 国家自然科学基金(No.81402025)资助
【分类号】：R574.62

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