胆固醇代谢相关蛋白及相关特异microRNAs在胆固醇型胆囊结石患者血清中的表达及意义

发布时间：2018-03-06 03:11

本文选题：胆固醇型胆囊结石病　切入点：胆固醇代谢　出处：《中国现代医学杂志》2016年13期 　论文类型：期刊论文

【摘要】：目的探讨胆固醇型胆囊结石患者和健康对照人群血清中胆固醇代谢相关蛋白,以及可能靶向ABCA1的相关micro RNAs(mi RNAs)表达差异及意义。方法选取60例胆固醇型胆囊结石患者(结石组)和60例健康对照人群(非结石组)的血清样本,采用Western blot检测胆固醇代谢相关蛋白(SREBP-2、FXR、LXR、ABCA1、ABCB11)的表达,利用生物信息学软件预测可能靶向调控ABCA1的相关mi RNAs,采用实时荧光定量聚合酶链反应(RT-q PCR)对相关mi RNAs的表达进行检测。结果结石组血清中SREBP-2、FXR、LXR、ABCA1、ABCB11蛋白的表达量较非结石组降低(P0.05),结石组血清中mi RNA-26a、mi RNA-144、mi RNA-758表达较非结石组升高(P0.05)。结论胆固醇代谢相关蛋白差异性表达及可能靶向ABCA1的相关mi RNA在胆固醇型胆囊结石的发病过程中可能发挥极为重要的作用。
[Abstract]:Objective to investigate the cholesterol gallstone patients and healthy controls serum cholesterol metabolism related protein, and may be targeted to ABCA1 micro RNAs (MI RNAs) expression and significance. Methods 60 cases of patients with cholesterol gallstone patients (Stone Group) and 60 healthy controls (non gallstone group) serum samples. Using Western blot to detect cholesterol metabolism related proteins (SREBP-2, FXR, LXR, ABCA1, ABCB11) expression, using bioinformatics software to predict the possible targeted regulation of ABCA1 mi RNAs by real-time fluorescent quantitative polymerase chain reaction (RT-q PCR) was used to detect the expression of MI RNAs. The results of SREBP-2, stone serum FXR, LXR, ABCA1, ABCB11 protein expression compared with non gallstone group decreased (P0.05), stone group serum mi RNA-26a, MI RNA-144, MI RNA-758 expression compared with non gallstone group increased (P0.05). Conclusion cholesterol on behalf of Xie Xiang The differential expression of the proteins and the associated mi RNA targeting ABCA1 may play an important role in the pathogenesis of cholesterol gallstone.

【作者单位】：贵州医科大学附属医院肝胆外科;
【基金】：国家自然科学基金(No:81560477) 贵州省科技计划项目[No:黔科合SY字(2015)3047]
【分类号】：R575.62

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