血管生成素及其他炎症介质在大鼠急性胆源性胰腺炎中的变化规律及意义

发布时间：2018-03-08 19:27

本文选题：胆源性胰腺炎　切入点：血管生成素　出处：《南京医科大学学报(自然科学版)》2017年01期 　论文类型：期刊论文

【摘要】：目的:探讨血管生成素及其他炎症介质在急性胆源性胰腺炎中的变化及其意义。方法:将70只SD大鼠随机分成急性胆源性胰腺炎组(ABP组,胆总管末端结扎,n=56)和假手术组(SO组,仅行开腹后翻转胰腺,n=14),分为7个时间点(术后0、6、12、24、48、72、120 h)。各组处理后于相应时间点留取大鼠的胰腺和静脉血,测得胰腺含水量,观察ABP组及SO组胰腺病理变化及评分并检测血中淀粉酶(AMY)、白介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)、核因子-κB(NF-κB)、血管生成素-1(Ang-1)、血管生成素-2(Ang-2)的变化。结果:ABP组胆总管末端结扎术后各时间点的AMY、IL-10、TNF-α、NF-κB均高于同时间点的SO组(P0.05),并于120 h达到观察期内峰值。Ang-1术后0 h即较SO组显著降低(P0.01),后升高并于12 h后保持平稳,但始终低于SO组(P0.05),与胰腺病理评分无相关性;Ang-2术后0 h即较SO组显著升高(P0.01),并于48 h到达峰值,且与胰腺病理评分呈正相关(r=0.943,P0.01),后缓慢下降但显著高于SO组,并失去与胰腺病理评分的正相关性。ABP组0~12 h,Ang-2与胰腺含水量呈正相关(r=0.830,P0.01),24 h后无明显相关性。ABP早期,胰腺含水量急剧升高与Ang-1的下降及Ang-2的升高相对应。结论:炎症介质在ABP的发生发展中起到重要作用,而Ang-1水平与ABP严重程度无明显相关性,Ang-2水平在ABP早期上升,峰值提前于其他炎症介质,与胰腺病理评分及胰腺含水量呈正相关,Ang-2可能在ABP早期起到促炎及促血管渗漏的作用,预示胰腺炎的严重程度,或可用于指导临床治疗。
[Abstract]:Objective: to investigate the changes and significance of angiopoietin and other inflammatory mediators in acute biliary pancreatitis. Methods: 70 SD rats were randomly divided into ABP group, ligation of choledochal end ligation of choledochus 56) and sham operation group. The rats were divided into 7 time points (0: 6, 12, 24, 48, 72, 120 hours after operation). The pancreas and venous blood were collected at the corresponding time points, and the pancreatic water content was measured. To observe the pathological changes and scores of pancreas in ABP group and so group, and to detect the changes of amylase, IL-10, TNF- 伪, NF- 魏 BNF-kappa, Ang-1 and Angiopoietin-2Ang-2 in blood of ABP group and so group. The AMYT IL-10 TNF- 伪 -NF- 魏 B at the time point was higher than that in the so group at the same time point (P 0.05), and reached the peak value within the observation period. Ang-1 at 0 h after operation was significantly lower than that in the so group at 0 h, and then increased and remained stable after 12 hours. However, it was always lower than that of so group (P 0.05), and had no correlation with pancreatic pathological score. It was significantly higher than that of so group at 0 h after Ang-2, and reached its peak at 48 h, and had a positive correlation with pancreatic pathological score (r 0.943 P 0.01), and then decreased slowly but significantly higher than that in so group. There was no significant correlation between Ang-2 and pancreatic water content in ABP group after 24 h. Conclusion: inflammatory mediators play an important role in the occurrence and development of ABP, but there is no significant correlation between the level of Ang-1 and the severity of ABP. The peak value was earlier than that of other inflammatory mediators, which was positively correlated with pancreatic pathological score and pancreatic water content. Ang-2 might play a role in promoting inflammation and promoting vascular leakage in early stage of ABP, indicating the severity of pancreatitis, and could be used to guide clinical treatment.
【作者单位】：南京医科大学鼓楼临床医学院消化科;
【基金】：南京市科技发展计划项目(201108022) 南京市卫生局杰出青年基金项目(JQX14005)
【分类号】：R576

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