天然提取与重组高迁移率组蛋白N2的抗乙型病毒性肝炎病毒活性比较

发布时间：2018-03-11 07:18

本文选题：人THP-细胞　切入点：高迁移率组蛋白N　出处：《中国临床药理学杂志》2015年05期 　论文类型：期刊论文

【摘要】：目的比较天然提取与重组高迁移率组蛋白N2(HMGN2)的抗乙型病毒性肝炎病毒(乙肝病毒,HBV)活性,以探讨HMGN2分子的抗HBV作用。方法以HBV DNA转染的细胞株Hep G 2.2.15细胞为体外抗HBV模型,用不同浓度的天然提取和重组HMGN2分子作用于Hep G 2.2.15细胞,分别在第4,8天收集细胞培养上清液,酶联免疫吸附(ELISA)法检测上清中HBV表面抗原(HBs Ag)及e抗原(HBe Ag),用实时荧光定量聚合酶链式反应(PCR)法检测上清液HBV DNA的含量。结果天然提取HMGN2与重组HMGN2在1~100mg·L-1对Hep G 2.2.15细胞无细胞毒性;天然提取HMGN2与重组HMGN2蛋白分子在1~5 mg·L-1水平即可显著抑制HBe Ag和HBs Ag的表达,可显著降低HBV DNA拷贝数,继续增加剂量,抗病毒效果不再明显增加,且在相同剂量下比较,两者作用差异无统计学意义。结论天然提取HMGN2与重组HMGN2分子在体外细胞培养中具有同样直接抗HBV活性。
[Abstract]:Objective to compare the antiviral hepatitis B virus (HBV) activity of naturally extracted and recombinant high mobility histone N2HMGN2, and to investigate the anti HBV effect of HMGN2. Methods the cell line Hep G2.2.15 transfected with HBV DNA was used as an in vitro anti-#en5# model. Hep G 2.2.15 cells were treated with different concentrations of natural extract and recombinant HMGN2 molecules. The supernatants of cell culture were collected on the 4th day. Enzyme linked immunosorbent assay (Elisa) was used to detect HBV surface antigen (HBs) and e antigen HBe AgN in supernatant. Real-time fluorescence quantitative polymerase chain reaction (PCR) method was used to detect the content of HBV DNA in supernatant. Results Natural HMGN2 and recombinant HMGN2 were extracted at 1 ~ 100mg 路L ~ (-1) and Hep G _ (2.2.15) 路L ~ (-1). There was no cytotoxicity. The expression of HBe Ag and HBs Ag could be significantly inhibited by natural extraction of HMGN2 and recombinant HMGN2 protein at the level of 1 渭 g 路L -1, and the copy number of HBV DNA was significantly decreased, and the antiviral effect was not significantly increased with the increase of the dosage of HBV DNA, and the antiviral effect was not significantly increased at the same dose. Conclusion Natural HMGN2 extraction and recombinant HMGN2 have the same direct anti-#en2# activity in cell culture in vitro.
【作者单位】：四川省医学科学院四川省人民医院药学部;
【基金】：中国科学院四川转化医学研究医院·四川省人民医院青年科学基金资助项目(30305030850)
【分类号】：R512.62

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