谷氨酰胺对梗阻性黄疸大鼠小肠黏膜组织Akt活性的影响

发布时间：2018-03-23 23:03

本文选题：梗阻性黄疸　切入点：小肠黏膜　出处：《山东医药》2015年36期

【摘要】：目的观察梗阻性黄疸大鼠小肠组织中蛋白激酶B(Akt)活性,探讨谷氨酰胺肠保护的作用机制。方法将64只SD大鼠随机分为4组各16只,模型组、甘氨酸组、谷氨酰胺组行梗阻性黄疸造模,假手术组游离上段胆总管后关腹;造模后1 d,甘氨酸组、谷氨酰胺组均给予同等热量的肠内营养剂灌胃,同时分别给予甘氨酸、谷氨酰胺1 g/(kg·d),模型组、假手术组给予等量生理盐水灌胃。各组分别于术后第3、7天处死大鼠8只,采用Western blotting法检测小肠组织Akt总蛋白及p-Akt,实时PCR检测肠组织Akt mRNA。结果与假手术组比较,模型组、甘氨酸组、谷氨酰胺组术后第3、7天小肠组织中Akt mRNA及p-Akt蛋白增加,且谷氨酰胺组高于模型组、甘氨酸组,P均0.05;模型组、甘氨酸组间比较,P均0.05。各组小肠组织Akt总蛋白比较,P均0.05。结论谷氨酰胺通过促进Akt信号通路的活化,发挥对小肠上皮细胞的保护作用。
[Abstract]:Objective to observe the activity of protein kinase (PKB) Akt in small intestine of rats with obstructive jaundice and to explore the protective mechanism of glutamine. Methods Sixty-four SD rats were randomly divided into 4 groups, 16 rats in each group, model group and glycine group. The rats in the glutamine group were treated with obstructive jaundice, and in the sham operation group, the upper common bile duct was closed, and the glycine group and the glutamine group were given the same caloric enteral nutrition at the same time as glycine. The rats in the model group and sham operation group were given the same amount of normal saline intragastrically. The rats in each group were killed on the 3rd day after operation, and 8 rats were killed on the 7th day after operation. Total Akt protein and p-Akt in small intestine were detected by Western blotting method, and Akt mRNA in intestinal tissue were detected by real-time PCR. Results compared with sham operation group, Akt mRNA and p-Akt protein in small intestine were increased in model group, glycine group and glutamine group on the 3rd day after operation. Glutamine group was higher than model group (P 0.05), model group (P 0.05), glycine group (P 0.05) and small intestine Akt protein (P 0.05). Conclusion glutamine can promote the activation of Akt signal pathway. To play a protective role on small intestinal epithelial cells.
【作者单位】：天津医科大学;天津市南开医院;
【基金】：国家自然科学基金资助项目(81273952)
【分类号】：R575

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