非酒精性脂肪性肝病患者和正常人群的唾液菌群的研究

发布时间：2018-03-28 16:44

本文选题：口腔菌群　切入点：NAFLD　出处：《实用医学杂志》2017年21期

【摘要】：目的分析非酒精性脂肪肝患者(NAFLD组)与正常对照组(HC组)唾液菌群的多样性。方法收集24例脂肪肝患者和22例正常志愿者的唾液标本,提取总DNA,用PCR扩增16S rDNAV4-V5区,并进行高通量测序,将测序数据进行OTU聚类、多样性分析及物种注释等。结果两组纳入对象的唾液菌群获得的序列平均有39 118条,其中NAFLD组聚类OTUs约308±48,HC组聚类OTUs约305±53。多样性分析NAFLD组和HC组示整体唾液菌群的多样性方面差异较小。物种分析数据表明唾液菌群在门及属水平的组成基本相同,但相对丰度不同,NAFLD组在门水平示厚壁菌门(20.42%)丰度比例升高,拟杆菌门(36.75%)比例降低;属水平的梭菌属、卟啉单胞菌属、韦永氏球菌属的比例较高,而普氏菌属、普雷沃菌属比例显著低。结论 NAFLD组相较于HC组的唾液中厚壁菌门的菌群丰度相对升高而拟杆菌门的菌群丰度相对减少,推测唾液菌群失衡与NAFLD发病可能存在一定关系。
[Abstract]:Objective to analyze the diversity of salivary flora in patients with nonalcoholic fatty liver (NAFLD) and control group (HC). Methods the total DNA was extracted from 24 patients with fatty liver and 22 healthy volunteers. The 16s rDNAV4-V5 region was amplified by PCR. High throughput sequencing was carried out. OTU clustering, diversity analysis and species annotation were performed on the sequencing data. Results the average number of sequences obtained from the two groups of salivary flora was 39,118. Among them, the OTUs of NAFLD group was about 308 卤48? HC group? OTUs was about 305 卤53. The diversity analysis of NAFLD group and HC group showed little difference in the diversity of the whole saliva flora. The species analysis data showed that the composition of salivary flora was basically the same at the phylum and genus level. But in NAFLD group with different relative abundance, the proportion of abundance increased and the proportion of Bacteroides 36.75) decreased at the gate level, while the proportion of Clostridium, porphyromonas, Wayonella was higher, while that of Pueraria was higher. Conclusion compared with HC group, the abundance of sputum of NAFLD group is higher than that of HC group, and the abundance of Bacteroides phylum is relatively lower. It is suggested that the imbalance of salivary flora may be related to the pathogenesis of NAFLD.
【作者单位】：南方医科大学;广东省人民医院广东省医学科学院消化内科;广东省人民医院体检中心;
【分类号】：R575.5

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