普拉梭菌上清对葡聚糖酸钠诱导的溃疡性结肠炎小鼠Th17细胞及IL-17A的影响

发布时间：2018-04-04 17:44

本文选题：普拉梭菌　切入点：溃疡性结肠炎　出处：《中南大学学报(医学版)》2015年12期

【摘要】：目的:探讨普拉梭菌(Faecalibacterium prausnitzii,Fp)上清对葡聚糖酸钠(dextran sulfate sodium,DSS)诱导的小鼠溃疡性结肠炎的预防和治疗作用及机制。方法:40只雄性C57BL/6J小鼠随机分为对照组、模型组、治疗组、预防组,每组10只。观察各组小鼠的结肠组织病理学评分,计算脾Th 17细胞占单核细胞比例;检测外周血浆白细胞介素-17A(interleukin-17A,IL-17A)和IL-6水平,以及结肠黏膜组织转录因子维甲酸相关孤儿核受体-γt(transcription factor retinoic acid-related orphan receptor-γt,ROR-γt),IL-17A和IL-6的m RNA表达情况。结果:治疗组和预防组小鼠结肠组织病理损伤较模型组轻,其病理组织学评分较模型组均明显下降(均P0.05),预防组和治疗组之间差异无统计学意义(P0.05);治疗组和预防组脾辅助性T细胞(helper T cell,Th17)比例较模型组均明显下降(均P0.01);治疗组血浆IL-17A和IL-6水平也较模型组均明显降低(均P0.05),结肠组织ROR-γt,IL-17A和IL-6的m RNA表达均明显低于模型组(均P0.05),但预防组血浆IL-6水平和结肠组织IL-6 m RNA表达与模型组差异无明显统计学意义(均P0.05)。结论:Fp上清可预防和治疗DSS所致的小鼠结肠炎,其机制可能为抑制脾和肠道Th 17细胞的生成,减少外周血和结肠组织炎性细胞因子IL-17A分泌;Fp上清可通过降低IL-6水平来治疗结肠炎。
[Abstract]:Objective: To investigate the effect of faecalibacterium prausnitzii (Faecalibacterium prausnitzii Fp) supernatant on dextran sodium (dextran sulfate, sodium, DSS) prevention and treatment effect and mechanism of induced ulcerative colitis in mice. Methods: 40 male C57BL/6J mice were randomly divided into control group, model group, treatment group, prevention group, 10 rats in each group. The score colon histopathology were observed in mice spleen Th cells accounted for 17, calculate the proportion of monocytes; detection of peripheral blood interleukin -17A (interleukin-17A, IL-17A) and the level of IL-6, and colonic mucosa of transcription factor retinoid related orphan nuclear receptor gamma t (transcription factor retinoic acid-related orphan receptor- y t. ROR- T, m RNA) gamma expression of IL-17A and IL-6. Results: the treatment group and prevention group mice colon tissue pathological damage was lighter than in the model group, the pathological score was lower than the model group were significantly decreased (P0.0 5), there was no significant difference between prevention group and treatment group (P0.05); treatment group and prevention group spleen helper T cells (helper T cell, Th17) compared with model group were significantly decreased (P0.01); treatment group, plasma IL-17A and IL-6 levels are lower than the model group were significantly reduced (P0.05) ROR-, colon tissue gamma T, m RNA and IL-17A IL-6 expression were significantly lower than those in the model group (P0.05), but the prevention of differential expression of plasma level of IL-6 and IL-6 RNA and m in colon tissue of model group was not statistically significant (P0.05). Conclusion: the mouse colitis Fp supernatant can prevent and treat DSS induced. The mechanism may be inhibiting formation of spleen and intestinal Th 17 cells, IL-17A reduced inflammatory cytokines in peripheral blood and colon tissue secretion; Fp supernatant to treat colitis by reducing the level of IL-6.

【作者单位】：南京医科大学鼓楼临床医学院消化科;东南大学附属南京江北人民医院消化科;南京大学医学院消化科;南京大学医学院附属鼓楼医院消化科;
【基金】：国家自然科学基金(81170559)~~
【分类号】：R574.62

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