青海地区藏、回族肝硬化患者血清瘦素水平及其基因C2549A多态性研究

发布时间：2018-04-06 05:33

本文选题：肝硬化　切入点：瘦素　出处：《实用医学杂志》2015年15期

【摘要】：目的:研究青海地区藏族和回族肝硬化患者血清瘦素水平及其与瘦素基因多态性的关系。方法:采用核素放射免疫法测定血清瘦素水平,聚合酶链反应-限制性片段长度多态性方法(PCR-RFLP)测定瘦素基因的多态性。结果:(1)藏族和回族肝硬化患者的血清瘦素水平都显著高于对照组(P0.01),两民族之间差异无显著性(P0.05)。(2)藏族和回族的男性肝硬化患者的血清瘦素显著高于对照组(P0.01),而女性肝硬化患者与对照组差异无显著性(P0.05)。(3)藏族和回族对照组女性AA+AC基因型的瘦素水平显著高于男性(P0.01),而CC基因型的男女瘦素水平差异无显著性(P0.05),观察组男女之间的AA+AC和CC基因差异无显著性(P0.05)。(4)两民族观察组和对照组的基因型间差异均无显著性(P0.05)。结论:青海地区藏族和回族乙肝肝硬化患者血清瘦素水平均高于对照组,并有性别差异;瘦素基因启动子2549位核苷酸存在多态性,两民族间差异无显著性。
[Abstract]:Objective: to study the relationship between serum leptin level and leptin gene polymorphism in Tibetan and Hui cirrhosis patients in Qinghai.Methods: serum leptin levels were measured by radionuclide radioimmunoassay and the polymorphism of leptin gene was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).Results the serum leptin levels of both Tibetan and Hui cirrhotic patients were significantly higher than those of the control group (P 0.01). There was no significant difference between the two nationalities in serum leptin levels in Tibetan and Hui male cirrhotic patients, while the serum leptin levels were significantly higher in Tibetan and Hui male cirrhotic patients than in the control group (P 0.01), while in female patients, the serum leptin levels were significantly higher than those in the control group (P < 0.01).The leptin level of AA AC genotype in Tibetan and Hui nationality control group was significantly higher than that in male (P 0.01), but there was no significant difference in leptin level between male and female in CC genotype. There was no significant difference in leptin level between male and female in observation group.There was no significant difference in the genotypes of AA AC and CC gene between the two ethnic groups.Conclusion: the levels of serum leptin in Tibetan and Hui patients with hepatitis B cirrhosis in Qinghai are higher than those in the control group, and there is a significant difference in leptin gene promoter 2549 nucleotide polymorphism between the two nationalities.
【作者单位】：青海大学附属医院;青海省第四人民医院;
【基金】：青海省科技厅基金资助项目(编号:2011-Z-731)
【分类号】：R575.2

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