低分子量促肝细胞生长素的提取及活性分析

发布时间：2018-04-10 07:47

本文选题：促肝细胞生长素　切入点：活性分析　出处：《南开大学学报(自然科学版)》2017年02期

【摘要】：采用膜过滤结合层析法从猪胎肝中提取纯化低分子量促肝细胞生长素,并采用MTT法,测定促肝细胞生长素对人肝癌细胞Hp G2的增殖影响,结果表明,8 k Da小肽在1.5和15μg/m L时可有效促进Hp G2的增殖,当浓度达到150μg/m L时,可抑制Hp G2的生长,该研究为肝病营养功能组件提供理论与技术基础,也为促细胞生长素的临床应用提供理论依据.
[Abstract]:Low molecular weight hepatocyte growth-promoting hormone (LMWHGF) was extracted and purified from porcine fetal liver by membrane filtration and chromatography. The effect of hepatocyte growth factor on the proliferation of human hepatoma cell line HpG2 was determined by MTT assay.The results showed that 8 kDa peptide could effectively promote the proliferation of HP G2 at 1.5 渭 g / mL and 15 渭 g / mL, and inhibit the growth of HP G2 when the concentration reached 150 渭 g / mL. This study provided a theoretical and technical basis for the nutritional functional components of liver diseases.It also provides a theoretical basis for the clinical application of auxin.
【作者单位】：北京(中驭)生物工程有限公司;
【基金】：国家高新技术研究发展计划(863计划)(2014AA022209)
【分类号】：R512.62;R575

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