替米沙坦对非酒精性脂肪肝大鼠肝组织PPARs及脂联素受体2表达的影响

发布时间：2018-04-12 00:12

本文选题：替米沙坦 + 非酒精性脂肪性肝病　；参考：《中国实验动物学报》2017年03期

【摘要】：目的探讨替米沙坦对非酒精性脂肪肝大鼠肝组织PPARs及脂联素受体2表达的影响。方法 40只雄性SD大鼠随机分为正常对照组(NC,n=15)、高脂对照组(FC,n=15)、高脂+替米沙坦干预组(FT,n=10)。NC组给予普通饲料,FC和FT组予高脂饲料喂养。12周末时随机取NC组和FC组各5只做正葡萄糖高胰岛素嵌夹实验和肝组织HE染色,确定造模成功后,FT组给予替米沙坦5 mg/(kg·d)灌胃,NC和FC组以等容量生理盐水灌胃,共4周。16周时应用高胰岛素正葡萄糖嵌夹实验技术稳态葡萄糖输注速率测定胰岛素敏感性,检测血清转氨酶、血脂及空腹血糖水平;应用逆转录聚合酶链反应法测定肝组织中过氧化物本酶体增殖活化受体(PPARs)、脂联素受体2(AdipoR2)和血管紧张素II 1型受体(AT1R)的表达。结果与NC组相比,FC组肝组织PPARα、PPARγ、AdipoR2 mRNA表达显著下降(P0.01),AT1R mRNA表达较NC组升高(P0.01);FT组PPARα、PPARγ、AdipoR2 mRNA表达较FC组升高(P0.01);FT组血清转氨酶、血脂及空腹血糖水平较FC组改善。结论替米沙坦能降低NASH大鼠的体重和肝指数,调节糖脂代谢并改善胰岛素抵抗状态,对NASH大鼠肝脏具有保护作用。
[Abstract]:Objective to investigate the effect of telmisartan on the expression of PPARs and adiponectin receptor 2 in liver tissue of rats with non-alcoholic fatty liver.Methods Forty male Sprague-Dawley rats were randomly divided into normal control group (n = 15), hyperlipidemia control group (n = 5), high fat telmisartan intervention group (n = 10) and FT group (n = 5).The normal glucose hyperinsulinic clamp test and HE staining of liver tissue were performed.It was determined that the FT group was given telmisartan for 5 mg/(kg d) and the FC group and the FC group were perfused with normal saline of the same volume. After 4 weeks and 16 weeks, the sensitivity of insulin was determined by the steady-state glucose infusion rate of hyperinsulinic positive glucose entrapment test.The levels of serum aminotransferase, serum lipids and fasting blood glucose were determined by reverse transcriptase polymerase chain reaction (RT-PCR). The expression of peroxisome proliferation-activated receptor (PPARsN), adiponectin receptor 2( AdipoR2) and angiotensin II type 1 receptor (AT1R) in liver tissue was determined by reverse transcriptase polymerase chain reaction (RT-PCR).Results compared with NC group, the expression of PPAR 伪 -PPAR- 纬 -PPAR- AdipoR2 mRNA in FC group was significantly lower than that in NC group. Compared with NC group, the expression of PPAR 伪 -PPAR- 伪 -PPAR- 纬 -PPAR- AdipoR2 mRNA in FC group was significantly higher than that in FC group, and the levels of serum lipid and fasting blood glucose were improved in FC group.Conclusion telmisartan can decrease the body weight and liver index of NASH rats, regulate the metabolism of glucose and lipid and improve the state of insulin resistance. Telmisartan has protective effect on the liver of NASH rats.
【作者单位】：河北医科大学第二附属医院消化内科;
【分类号】：R575.5

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