酪酸梭菌、美沙拉嗪、蒙脱石散对溃疡性结肠炎大鼠血IL-6、IL-8、IL-10及TNF-α水平的影响

发布时间：2018-04-15 01:35

本文选题：酪酸梭菌 + 美沙拉嗪　；参考：《免疫学杂志》2015年11期

【摘要】：目的了解酪酸梭菌、美沙拉嗪、蒙脱石散对TNBS/乙醇法诱导的溃疡性结肠炎(UC)大鼠IL-6、IL-8、IL-10及TNF-α表达的影响,为临床治疗UC提供新的依据。方法 120只SD大鼠随机分为正常对照组、模型组、酪酸梭菌、美沙拉嗪组、蒙脱石散组、美沙拉嗪联合蒙脱石散组共6组,正常对照组不给任何处理,其余各组通过TNBS/乙醇法建立UC的大鼠模型,24 h后每日分别灌服相应剂量生理盐水、酪酸梭菌、美沙拉嗪、蒙脱石散、美沙拉嗪联合蒙脱石散干预治疗,治疗满5 d、12 d时各抽取一半大鼠,收集血标本及结肠标本,HE染色观察结肠病理改变,ELISA法测定血清中IL-6、IL-8、IL-10、TNF-α的含量。结果与正常对照组比较,模型组、酪酸梭菌组、美沙拉嗪组、蒙脱石散组、美沙拉嗪联合蒙脱石散组的DAI评分及血清IL-6、IL-8、TNF-α含量均升高,血清IL-10含量均降低,差异有统计学意义(P0.05)。与模型组比较,酪酸梭菌组、美沙拉嗪组、蒙脱石散组、美沙拉嗪联合蒙脱石散组的DAI评分及血清IL-6、IL-8、TNF-α含量均降低,血清IL-10含量均升高,差异有统计学意义(P0.05),同时结肠组织病理改变明显减轻。酪酸梭菌、美沙拉嗪、蒙脱石散、美沙拉嗪联合蒙脱石散的治疗效果比较,美沙拉嗪和美沙拉嗪联合蒙脱石散疗效较好(P0.05),酪酸梭菌与蒙脱石散疗效差异不明显。结论 TNBS/乙醇法诱导的实验性UC大鼠血清IL-6、IL-8、TNF-α表达增高,与炎症呈正相关;IL-10表达降低,与炎症呈负相关。酪酸梭菌、美沙拉嗪、蒙脱石散可能通过下调UC大鼠促炎因子IL-6、IL-8、TNF-α的分泌,上调抗炎因子IL-10的分泌,减轻炎症反应,促进肠黏膜组织的修复。
[Abstract]:Objective to investigate the effects of Clostridium caseate, mesalazine and montmorillonite powder on the expression of IL-6, IL-8, IL-10 and TNF- 伪 in rats with ulcerative colitis induced by TNBS/ ethanol, and to provide a new basis for clinical treatment of UC.Methods 120 Sprague-Dawley rats were randomly divided into 6 groups: normal control group, model group, Clostridium butyricum group, mesalazine group, montmorillonite powder group and mesalazine combined with montmorillonite powder group.The other groups were given corresponding doses of normal saline, Clostridium butyrate, mesalazine, montmorillonite powder, mesalazine combined with montmorillonite powder for 24 h after establishment of UC rat model by TNBS/ ethanol method.Blood samples and colon specimens were collected to observe the pathological changes of colon and Elisa method was used to detect the content of IL-8 and IL-10 TNF- 伪 in serum.Results compared with the normal control group, the DAI scores and serum IL-6 / IL-8 TNF- 伪 levels in the model group, Clostridium butyrate group, mesalazine group, montmorillonite powder group and mensalazine combined with montmorillonite powder group were increased, and the serum IL-10 levels were decreased (P 0.05).Compared with the model group, the DAI scores and the serum IL-6 / IL-8 TNF- 伪 levels in the clostridium caseate group, mesalazine group, montmorillonite powder group, mecalazine combined with montmorillonite powder group were decreased, and the serum IL-10 content was increased.The difference was statistically significant (P 0.05), and the pathological changes of colon were alleviated.The therapeutic effects of clostridium caseate, mesalazine, montmorillonite powder and mesalazine combined with montmorillonite powder were better than that of clostridium casalazine and montmorillonite powder, but there was no significant difference between clostridium caseate and montmorillonite powder.Conclusion the expression of IL-6, IL-8 and TNF- 伪 in the serum of experimental UC rats induced by TNBS/ ethanol was increased, which was positively correlated with inflammation and negatively correlated with inflammation.Clostridium caseate, mesalazine and montmorillonite may down-regulate the secretion of IL-6 and IL-8 TNF- 伪, up-regulate the secretion of anti-inflammatory factor IL-10, alleviate inflammatory reaction and promote the repair of intestinal mucosa.
【作者单位】：云南省第一人民医院儿科;昆明医科大学第一附属医院儿科;
【基金】：益普生腹泻基金(IDF-2012-01)
【分类号】：R574.62

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