高热量饲料结合慢性应激致大鼠非酒精性脂肪肝病变过程的研究

发布时间：2018-04-17 11:39

本文选题：高热量饲料 + 慢性应激　；参考：《安徽农业大学学报》2015年02期

【摘要】：为观察不同作用时间下非酒精性脂肪肝(NAFLD)相关因子的改变,研究高热量饲料结合慢性应激促进NAFLD病变的过程,将64只雄性大鼠分为正常对照组、高热量饲料(HFSD)组、慢性应激(EFNS)组及高热量饲料结合慢性应激(HFSD+EFNS)组。8周后,每组随机取8只动物,作为第1批实验动物;14周后,余下每组8只动物作为第2批实验动物,检测胰岛素抵抗、血液及肝组织脂质、氧化应激状态、肿瘤坏死因子α(TNF-α)及基因表达,计算肝指数。另外,取肝脏做病理切片,比较各组肝组织病理改变程度。结果显示,8周后大鼠出现胰岛素抵抗、脂质紊乱、氧化应激、炎症状态及肝指数增加。2种刺激只对肝脂质代谢紊乱有交互作用。14周后,大鼠NAFLD进一步恶化,对脂质紊乱、肝脂质代谢、炎症、氧化应激状态均有协同作用,肝组织切片也显示出现脂肪性肝炎。高热量饲料结合慢性应激促进NAFLD有一个发展过程,14周后能够诱导大鼠非酒精性脂肪肝模型的形成。
[Abstract]:In order to observe the changes of NAFLD- related factors in non-alcoholic fatty liver at different time, and to study the process of hypercaloric diet combined with chronic stress to promote NAFLD lesion, 64 male rats were divided into normal control group and high calorie diet group.After 8 weeks, 8 animals in each group were randomly selected as the first batch of experimental animals, and the remaining 8 animals in each group were used as the second batch of experimental animals to detect insulin resistance.Lipid in blood and liver tissue, oxidative stress state, TNF- 伪) and gene expression, liver index were calculated.In addition, liver sections were taken to compare the pathological changes of liver tissues in each group.The results showed that insulin resistance, lipid disorder, oxidative stress, inflammatory state and liver index increased in rats after 8 weeks.Liver lipid metabolism, inflammation and oxidative stress all had synergistic effects. Liver tissue sections also showed fatty hepatitis.High calorie diet combined with chronic stress could induce the formation of non-alcoholic fatty liver model in rats after 14 weeks of development of NAFLD.
【作者单位】：哈励逊国际和平医院药学部;
【基金】：哈励逊国际和平医院研究生科研基金项目(2014-06)资助
【分类号】：R575.5

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