虾青素对小鼠肝脏原代细胞氧化应激的影响

发布时间：2018-04-17 13:16

本文选题：小鼠肝脏原代细胞 + 虾青素　；参考：《华南农业大学学报》2017年06期

【摘要】：【目的】研究虾青素对过氧化氢(H_2O_2)诱导小鼠肝脏原代细胞产生氧化应激的影响。【方法】原位二步灌流法提取ICR小鼠的肝脏原代细胞,用丙二醛(Malondialdehyde,MDA)作为指标、采用H_2O_2处理建立氧化应激模型。采用流式细胞仪测定细胞中活性氧(Reative oxygen species,ROS)含量及凋亡水平变化,生物化学法测定细胞中MDA和谷胱甘肽(Glutathione,GSH)的含量、超氧化物歧化酶(Superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)的活性,用荧光定量PCR检测抗氧化酶SOD、GSH-px mRNA的相对表达量,Western-blot检测细胞核中Nrf2蛋白相对表达量。【结果】使用5μg·mL~( 1)虾青素预保护细胞3 h,10μmol·L 1H_2O_2刺激3 h的情况下氧化应激模型效果最好。虾青素降低了H_2O_2诱导后小鼠肝脏原代细胞的细胞凋亡率以及ROS、MDA和GSH含量,提升了SOD、GSH-px的活性以及SOD、GSH-px的mRNA相对表达量,抑制了Nrf2蛋白的核转移。【结论】虾青素对H_2O_2诱导产生氧化应激的肝脏原代细胞具有保护作用。
[Abstract]:[objective] to study the effect of astaxanthin on oxidative stress in primary mouse liver cells induced by Astaxanthin. [methods] Primary liver cells of ICR mice were extracted by in situ two-step perfusion, and malondialdehyde (malondialdehyde) malondialdehyde (malondialdehyde) was used as an index.Oxidative stress model was established by H_2O_2 treatment.The contents of MDA and Glutathione glutathione (GSH), the activities of superoxide dismutase (SOD) and glutathione peroxidase (Glutathione peroxidase) and the activities of glutathione peroxidase (Glutathione peroxidase) and glutathione peroxidase (Glutathione peroxidase) were measured by flow cytometry.The relative expression of GSH-px mRNA was detected by fluorescence quantitative PCR and the relative expression of Nrf2 protein in the nucleus was detected by Western-blot. [results] the model of oxidative stress was best stimulated with 5 渭 g / mL astaxanthin (10 渭 mol / L) 1H_2O_2 for 3 h.Astaxanthin decreased the apoptosis rate, the content of rosbane MDA and GSH, increased the activity of GSH-px and the relative expression of GSH-px in H_2O_2 induced primary mouse liver cells.[conclusion] astaxanthin has protective effect on hepatic primary cells induced by H_2O_2 induced oxidative stress.
【作者单位】：吉林农业大学动物科学技术学院;吉林农业大学中药材学院;
【基金】：国家自然科学基金(31672511)
【分类号】：R575

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