磷酸腺苷激活的蛋白激酶在慢性应激诱发小鼠非酒精性脂肪肝中的作用

发布时间：2018-04-19 14:30

本文选题：非酒精性脂肪肝 + 应激　；参考：《第二军医大学学报》2017年12期

【摘要】：目的探讨磷酸腺苷激活的蛋白激酶(AMPK)及其激动剂5-氨基咪唑-4-甲酰胺核苷酸(AICAR)在慢性应激诱发小鼠非酒精性脂肪肝(NAFLD)中的作用。方法建立小鼠慢性应激模型,设对照组、应激组、应激加AICAR给药组和AICAR给药组,每组6只。采用ELISA法检测小鼠血浆促炎性细胞因子(肿瘤坏死因子α,γ干扰素)浓度,采用自动生化分析仪检测小鼠血浆丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、总胆固醇、三酰甘油、游离脂肪酸的水平,采用苏木精-伊红染色和油红O染色检测肝细胞脂肪变性,采用蛋白质印迹法检测肝组织AMPK蛋白表达;然后用AICAR处理慢性应激小鼠并检测上述指标的变化。结果慢性应激导致小鼠肝细胞脂肪变性,肝功能损害(ALT、AST水平升高,P0.01),血浆促炎性细胞因子浓度升高(P0.05),血浆游离脂肪酸水平升高(P0.01)以及肝组织AMPK蛋白表达降低(P0.01)。AICAR改善了肝细胞脂肪变性,并缓解了上述指标的变化。结论慢性应激可能通过AMPK信号通路诱发NAFLD,AMPK激动剂AICAR可缓解慢性应激导致的NAFLD。
[Abstract]:Objective to investigate the role of adenosine phospho-activated protein kinase (AMPK) and its agonist 5-aminoimidazole-4-formamide nucleotides (AICARA) in chronic stress-induced NAFLDs of non-alcoholic fatty liver in mice.Methods the chronic stress model was established in mice. There were 6 rats in each group: control group, stress group, stress plus AICAR group and AICAR group.The plasma levels of pro-inflammatory cytokines (tumor necrosis factor 伪, interferon 纬) in mice were detected by ELISA method. The plasma alanine aminotransferase (alt), aspartate aminotransferase (AST), total cholesterol (TC), triglyceride (TG) were detected by automatic biochemical analyzer.The levels of free fatty acids were detected by hematoxylin eosin staining and oil red O staining respectively. The expression of AMPK protein in liver tissue was detected by Western blotting and then AICAR was used to treat chronic stress mice and to detect the changes of the above indexes.Results chronic stress induced fatty degeneration of hepatocytes in mice.AICAR improved hepatic steatosis and ameliorated the changes of hepatic steatosis by increasing the level of AST, increasing the concentration of proinflammatory cytokines and free fatty acids (P0.05 and P0.01), and decreasing the expression of AMPK protein in liver tissue (P 0.01). AICAR improved the fatty degeneration of hepatocytes and alleviated the changes of the above indexes.Conclusion chronic stress may induce NAFLDK AMPK agonist AICAR through AMPK signaling pathway to alleviate NAFLD induced by chronic stress.
【作者单位】：第二军医大学东方肝胆外科医院肝外五科;第二军医大学学员旅学员3队;第二军医大学国际交流学院;
【分类号】：R575.5

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