IL-28B基因多态性与丙型肝炎自然转归的相关性研究

发布时间：2018-04-20 07:51

本文选题：丙型肝炎病毒 + IL-B　；参考：《中国免疫学杂志》2015年04期

【摘要】：目的:探讨IL-28B单核苷酸多态性与丙型肝炎病毒(HCV)感染自然转归的相关性。方法:采用Sanger基因测序法检测广州地区无偿献血人群中266名HCV感染者(107名自发清除者和159名慢性感染者)和97名健康献血者IL-28B rs12979860位点基因型和等位基因,并统计分析其在HCV感染者与健康献血者,HCV慢性感染者与健康献血者,HCV自发清除者与慢性感染者中的分布差异。结果:159名HCV慢性感染者,107名自发清除HCV者和97名健康献血者多表现为CC基因型,分别占83.6%、95.3%和86.6%,CT基因型分别占16.4%、4.7%和13.4%,未发现TT基因型。HCV感染者和健康献血者,HCV慢性感染者和健康献血者CC/CT基因型分布差异均无统计学意义(χ2=0.204,P=0.652;χ2=0.406,P=0.524),但在HCV慢性感染者和自发清除HCV者中CC/CT基因型分布差异有统计学意义(χ2=8.474,P=0.004),自发清除组中CC基因型比率高于慢性感染组,自发清除组C等位基因的频率高于慢性感染组(χ2=7.949,P=0.005)。结论:在广州献血人群中,IL-28B rs12979860基因多态性与HCV的易感性不相关,但与HCV感染的自发清除相关,表现为C等位基因有利于丙型肝炎病毒(HCV)的自发清除。
[Abstract]:Objective: To investigate the correlation between the single nucleotide polymorphism of IL-28B and the natural prognosis of hepatitis C virus (HCV) infection. Methods: the Sanger gene sequencing method was used to detect the genotype and allele of 266 HCV infected persons (107 spontaneous and 159 chronic) and 97 healthy blood donors in Guangzhou free blood donors. The distribution difference between HCV infected people and healthy blood donors, HCV chronic infected and healthy donors, HCV spontaneous scavengers and chronic infected persons was statistically analyzed. Results: 159 HCV chronic infected persons, 107 spontaneous HCV and 97 healthy blood donors were the CC genotypes, accounting for 83.6%, 95.3% and 86.6%, CT genotypes, respectively. Do not occupy 16.4%, 4.7% and 13.4%. No TT genotype.HCV infection and healthy donors were found. There was no significant difference in the distribution of CC/CT genotype between the HCV chronic infection and the healthy donors (x 2=0.204, P=0.652; Chi 2=0.406, P=0.524), but there was a statistical difference between the HCV chronic infection and the spontaneous clearance of HCV (chi square) (x 2=8.47). 4, P=0.004), the ratio of CC genotype in spontaneous clearance group was higher than that of chronic infection group, and the frequency of C allele in spontaneous clearance group was higher than that of chronic infection group (x 2=7.949, P=0.005). Conclusion: in Guangzhou blood donors, the polymorphism of IL-28B rs12979860 gene is not related to the susceptibility of HCV, but it is associated with the spontaneous clearance of HCV infection, which is a C allele. It is beneficial to the spontaneous clearance of hepatitis C virus (HCV).

【作者单位】：广州医科大学研究生学院;广州血液中心;
【基金】：国家自然科学基金(81273145) 广东省自然科学基金项目(S2013010015459) 广州市医药卫生科技重大项目(20141A031001)资助
【分类号】：R512.63

【参考文献】