MicroRNA-21抑制淫羊藿素诱导肝星状细胞凋亡的研究

  本文关键词：MicroRNA-21抑制淫羊藿素诱导肝星状细胞凋亡的研究，由笔耕文化传播整理发布。

        目的探讨microRNA-21在淫羊藿素诱导人肝星状LX-2细胞凋亡中的作用和机理。方法1、DAPI染色后荧光显微镜及Annexin V/PI双染色后行流式细胞术检测淫羊藿素刺激LX-2细胞后的凋亡。2、定量PCR检测microRNA-21的表达。3、瞬时细胞转染pre-microRNA-21和anti-microRNA-21调节microRNA-21的表达。4、Western blot观察凋亡相关蛋白Bcl-2、Bax的表达。结果1、淫羊藿素剂量依赖性诱导LX-2细胞的凋亡(P<0.05)2、淫羊藿素剂量依赖性减低LX-2细胞microRNA-21的表达(P<0.05)3、上调microRNA-21的表达减少淫羊藿素诱导的LX-2细胞的凋亡(P<0.05),而下调microRNA-21的表达则增加细胞凋亡(P<0.05)4、淫羊藿素下调LX-2细胞Bcl-2蛋白的表达,上调Bax蛋白的表达。而microRNA-21部分阻断了这种作用。结论MicroRNA-21介导了淫羊藿素诱导的肝星状细胞凋亡。

    ObjectiveTo investigate the role of microRNA-21in icaritin-induced apoptosis of hepatic stellate LX-2cell and possible mechanism involved.Methods1.Detection of the apoptotic cells by4’,6-diamidino-2-phenylindole and flow cytometry.2.Real-time polymerase chain reaction was used to detect microRNA-21expression changes.3.Transient transfection with pre-microRNA-21and anti-microRNA-21to regulate microRNA-21expression.4. Western blot was used to examine the protein expression of Bcl-2and Bax.Results1.Icaritin dose-dependently increased the apoptotic of LX-2cells (P<0.05)2.Icaritin dose-dependently decreased the expression of microRNA-21in LX-2cells (P<0.05)3.Overexpression of microRNA-21reduced icaritin-induced LX-2cells apoptosis (P<0.05),but inhibition of microRNA-21expression increased icaritin-induced LX-2cells apoptosis(P<0.05).4.Icaritin downregulated the protein expression of Bcl-2and upregulated the protein expression of Bax in LX-2cells.MicroRNA-21partly reversed the protein expression of Bcl-2and Bax induced by icaritin.ConclusionsMicroRNA-21mediates icaritin-induced LX-2cells apoptosis.

MicroRNA-21抑制淫羊藿素诱导肝星状细胞凋亡的研究

英文缩略词表5-7中文摘要7-8Abstract8引言9-15前言15材料和方法15-25结果25-32讨论32-34小结34-35结论35-36参考文献36-43综述43-53    参考文献50-53致谢53-54

  本文关键词：MicroRNA-21抑制淫羊藿素诱导肝星状细胞凋亡的研究，由笔耕文化传播整理发布。