RGDyK环肽对肝星状细胞增殖和迁移的影响

发布时间：2018-04-20 19:42

本文选题：RGD环肽 + 肝星状细胞　；参考：《中国临床解剖学杂志》2015年01期

【摘要】：目的探讨RGDyK环肽对活化肝星状细胞中整合素αvβ3表达和细胞迁移的影响。方法分离SD大鼠肝星状细胞,体外传代培养诱导细胞活化,用人工合成的整合素αvβ3特异配基RGDy K环肽处理肝星状细胞,MTT法计算其生长抑制率,通过免疫化学和荧光染色,RT-PCR和Western Blot分析,与对照组比较肝星状细胞中整合素αvβ3和α-SMA的表达变化,划痕实验观察RGDy K环肽对活化肝星状细胞迁移的影响。结果与对照组相比,RGDyK环肽抑制肝星状细胞增殖并有明显的量效关系,RGDyK环肽下调肝星状细胞中整合素αvβ3和α-SMA的在mRNA和蛋白质水平的表达(P0.05),降低肝星状细胞的迁移率(P0.01)。结论 RGDyK环肽抑制肝星状细胞增殖、收缩和迁移等生物行为与下调活化肝星状细胞整合素αvβ3表达有关。
[Abstract]:Objective to investigate the effect of RGDyK cyclic peptide on integrin 伪 v 尾 3 expression and cell migration in activated hepatic stellate cells. Methods Hepatic stellate cells from SD rats were isolated and cultured in vitro to induce cell activation. The growth inhibition rate of hepatic stellate cells was calculated by using synthetic integrin 伪 v 尾 3 specific ligand RGDy K cyclopeptide. The expression of integrin 伪 v 尾 3 and 伪 -SMA in hepatic stellate cells was analyzed by immunochemistry and fluorescence staining RT-PCR and Western Blot. The effect of RGDy K cyclopeptide on the migration of activated hepatic stellate cells was observed by scratch assay. Results compared with the control group, RGDyK cyclopeptide inhibited the proliferation of hepatic stellate cells in a dose-dependent manner. RGDyK cyclopeptide down-regulated the expression of integrin 伪 v 尾 3 and 伪 -SMA in hepatic stellate cells at mRNA and protein levels, and decreased the migration rate of hepatic stellate cells. Conclusion RGDyK cyclic peptide inhibits the proliferation, contraction and migration of hepatic stellate cells by down-regulating the expression of integrin 伪 v 尾 3 in activated hepatic stellate cells.
【作者单位】：昆明理工大学附属医院云南省第一人民医院消化科;
【基金】：国家自然科学基金(81160062) 云南省科技计划项目(2011FZ283)
【分类号】：R575.2

【共引文献】