色氨酸缓解甲氨蝶呤引起的大鼠肠道损伤

发布时间：2018-04-24 01:18

本文选题：色氨酸 + 肠道损伤　；参考：《南昌大学》2014年硕士论文

【摘要】：本试验评价了色氨酸对由甲氨蝶呤（MTX）引起大鼠损伤的肠道形态结构、抗氧化能力和机体代谢的影响。通过灌胃不同剂量色氨酸（Trp），分析大鼠血生化指标、氧化应激相关指标、血红素氧合酶-1和内源性代谢物等的变化，探讨色氨酸对肠道损伤的保护作用。方法：选用SD雄性大鼠，体重为200～220g。大鼠预饲一周后按体重随机分为6组，每组8只：正常对照组（NC组）、色氨酸对照组（TRP组）、甲氨蝶呤模型组（MTX组）、低剂量色氨酸+甲氨蝶呤组（L-TRP+MTX组）、中剂量色氨酸+甲氨蝶呤组（M-TRP+MTX组）、高剂量色氨酸+甲氨蝶呤组（H-TRP+MTX组）。预饲一周后，不同剂量色氨酸干预组大鼠分别灌胃等体积的100、200和300mg/kg.bw的色氨酸；正常对照组和色氨酸对照组分别灌喂等体积的蒸馏水和200mg/kg.bw的色氨酸，每日按剂量灌胃一次，连续7天；第4天静脉注射单剂量甲氨蝶呤（10mg/kg.bw），连续两天，，对照组注射等体积的生理盐水。实验结果：(1)色氨酸能改善肠道形态结构，其中低、中剂量色氨酸的效果更显著（P0.05）；(2)色氨酸使血浆中D-乳酸含量降低（P0.05）；(3)氧化应激导致大鼠肠组织氧化还原状态失衡，使MDA和MPO水平显著增加（P0.01）；色氨酸有助于提高其抗氧化能力，低、中剂量色氨酸对于提高抗氧化能力效果更显著(P0.05)；(4)通过色氨酸干预能显著提高HO-1的表达，其中低、中剂量色氨酸干预效果更好（P0.05）；(5)氧化应激导致机体代谢紊乱，色氨酸使大鼠血清中葡萄糖和脯氨酸含量降低，琥珀酸和柠檬酸含量升高（P0.05）。结论：色氨酸能改善肠道形态结构，提高组织抗氧化能力，调节机体代谢。色氨酸能有效地保护组织器官免受氧化损伤，这可能与HO-1的表达有关。
[Abstract]:The effects of tryptophan on the intestinal morphology, antioxidant capacity and metabolism of rats induced by methotrexate (MTX) were evaluated. The changes in blood biochemical indexes, oxidative stress related indexes, heme oxygenase -1 and endogenous metabolites were analyzed by different doses of tryptophan (Trp), and tryptophan was used to investigate the effect of tryptophan on intestine. Protective effect of canal injury.
Methods: SD male rats were divided into 6 groups according to weight of 200 ~ 220g. rats, each group was divided into 6 groups according to weight, 8 rats in each group: normal control group (group NC), tryptophan control group (group TRP), methotrexate group (MTX group), low dose tryptophan + methotrexate group (L-TRP+ MTX group), middle dose tryptophan + methotrexate group (M-TRP+MTX group), high dose Tryptophan + methotrexate group (group H-TRP+MTX). After a week of prefeeding, 100200 and 300mg/kg.bw tryptophan of different doses of tryptophan intervention group were given respectively. The normal control group and tryptophan control group were fed with equal volume of distilled water and tryptophan 200mg/kg.bw, respectively, once daily for 7 days at a dose, and fourth days. Intravenous injection of single dose of methotrexate (10mg/kg.bw) for two consecutive days, the control group was injected with equal volume of normal saline.
The results were as follows: (1) tryptophan could improve the morphological structure of intestinal tract, of which low dose tryptophan was more effective (P0.05); (2) tryptophan decreased the content of D- lactate (P0.05) in plasma (P0.05); (3) oxidative stress caused the imbalance of redox state of intestinal tissue in rats and increased the level of MDA and MPO significantly (P0.01); tryptophan helped to improve its oxygen resistance. The effect of tryptophan is more significant (P0.05) in improving the antioxidant capacity (P0.05); (4) the intervention of tryptophan can significantly improve the expression of HO-1, which is lower, the middle dose of tryptophan is better (P0.05); (5) oxidative stress causes metabolic disorder in the body, and tryptophan reduces the content of glucose and proline in the serum of rats, succinic The content of perperic acid and citric acid increased (P0.05).
Conclusion: tryptophan can improve the morphological structure of the intestine, improve the antioxidant capacity of the tissue and regulate the metabolism of the body. Tryptophan can protect the tissues and organs from oxidative damage effectively, which may be related to the expression of HO-1.

【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R574

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