自噬在实验性急性胰腺炎中变化的研究

发布时间：2018-04-27 15:43

本文选题：自噬 + 急性胰腺炎　；参考：《南昌大学》2014年硕士论文

【摘要】：目的：探讨自噬在实验性急性胰腺炎中的变化，通过观察自噬在急性胰腺炎发病过程的变化情况，说明自噬与急性胰腺炎的关系，进一步为急性胰腺炎的发病机制提供新的理论。方法：将SD大鼠54只，按照随机分配原则将大鼠分为三组，对照组（SO），急性水肿性胰腺炎（AEP）,急性坏死性胰腺炎（ANP），采用胰胆管逆行注射牛磺胆酸钠方法制造模型。每组大鼠按不同的时相点分别于术后第3、6、12h麻醉后心脏采血，留取血清检测淀粉酶；并将胰腺组织小部分做电镜检测；小部分用4%甲醛固定，常规切片和HE染色；采用免疫组化方法检测LC3B、P62表达。结果： 1、胰腺病理学评分： ①AEP组、ANP组和SO组之间各时间点病理损伤评分差异有显著性（P0.05）；②AEP组病理损伤评分开始增加，6h与12hAEP组无显著差异（P0.05）；③ANP组损伤评分明显增加，随时间增加损伤程度增加，各时间点差异有显著性（P0.05）。 2、血清淀粉酶变化： ①SO组各时间点之间血淀粉酶值无显著差异（P0.05）；②AEP组、ANP组较SO组值有升高，ANP组中较AEP组升高，且差异有显著性（P0.05）。 3、胰腺组织电镜下表现：我们观察3h各组电镜下的表现:3hAEP组和3hANP组较SO组自噬体和自噬溶酶体数量明显增加（P0.05），，3hANP组较3hAEP组自噬体和自噬溶酶体数量明显增加（P0.05）。 4、胰腺组织LC3B免疫组化染色评分： ①SO组可见LC3B少量表达，且各时间点无显著差异（P0.05）；AEP组、ANP组LC3B表达增强，与SO组比较差异有显著性（P0.05）；②AEP组中LC3B表达在6h和12h表达开始明显增加，与3h比较差异有显著性（P0.05）。③ANP组LC3B表达明显增加，染色加深，且随着时间的延长，表达逐渐增加，与AEP组同一时间点比较差异有显著性（P0.05）。 5、胰腺组织P62免疫组化染色评分： ①SO组见少量P62表达，且各时间点无明显差异（P0.05）；AEP组、ANP组P62表达增强，与SO组比较有差异显著性（P0.05）；②但同一时间点AEP组与ANP组间P62表达无显著差异性，AEP组和ANP组中各时间点之间无显著差异（P0.05）。结论： 1、经胰胆管逆行注射1%、5%牛磺胆酸钠成功复制了AEP和ANP动物模型。 2、AEP组和ANP组中自噬的表达是增加的，且ANP组中自噬的表达较AEP组中表达增加，ANP组和AEP组中自噬的表达随病理损伤加重而增加。 3、AEP组和ANP组中自噬的降解水平减弱。 4、自噬的功能损伤可能在急性胰腺炎的发病过程中起着一定作用。
[Abstract]:Objective: To explore the changes of autophagy in experimental acute pancreatitis, and to explain the relationship between autophagy and acute pancreatitis by observing the changes of autophagy in the pathogenesis of acute pancreatitis, and to provide a new theory for the pathogenesis of acute pancreatitis. Methods: Fifty-four SD rats were divided into three groups according to the principle of random distribution: control group, acute edematous pancreatitis (AEPN) and acute necrotizing pancreatitis (ANPN). The model was established by retrograde injection of sodium taurocholate into the pancreatic duct. In each group, the blood samples were collected from the heart at different time points at 6h after anesthesia, and the serum amylase was collected, and a small portion of pancreatic tissue was examined by electron microscopy, and a small portion was fixed with 4% formaldehyde, and stained with routine sections and HE. Immunohistochemical method was used to detect the expression of LC _ 3B ~ (2 +) P _ (62). Results: 1. Pancreatic pathological score: There was significant difference in pathological injury score between 1AEP group and so group at different time points. There was no significant difference in pathological injury score between 1AEP group and 12hAEP group at 6 h. There was significant difference between different time points (P 0.05). 2, the changes of serum amylase: There was no significant difference in serum amylase value between different time points in 1SO group. The serum amylase level in AEP group was higher than that in so group. The level of amylase in ANP group was higher than that in AEP group, and the difference was significant (P 0.05). 3. The histologic features of the pancreas were as follows: At 3 h, the number of autophagy and lysosome in AEP group and 3hANP group were significantly increased than those in so group. The number of autophagy and lysosome in 3hAEP group was significantly higher than that in 3hAEP group. 4. LC3B immunohistochemical staining score of pancreatic tissue: A small amount of LC3B expression was observed in the 1SO group, and there was no significant difference at each time point. The LC3B expression in the 1SO group increased significantly compared with that in the so group, and the expression of LC3B began to increase at 6 and 12 hours. Compared with 3h, the expression of LC3B in P0.05An 路3ANP group was obviously increased, the staining was deepened, and the expression increased gradually with the prolongation of time, and there was a significant difference between P0.05ANP group and AEP group at the same time point. 5, P62 immunohistochemical staining score of pancreatic tissue: A small amount of P62 expression was found in 1SO group, and there was no significant difference at each time point. There was no significant difference in the expression of P62 between AEP group and ANP group at the same time point. There was no significant difference between AEP group and ANP group at different time points. Conclusion: 1. The animal models of AEP and ANP were successfully established by retrograde injection of 5% sodium taurocholate through the pancreaticobiliary duct. 2the expression of autophagy was increased in AEP group and ANP group, and the expression of autophagy in ANP group was higher than that in AEP group. 3 the degradation level of autophagy in AEP group and ANP group was decreased. 4, the function of autophagy may play a role in the pathogenesis of acute pancreatitis.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R576

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