生长激素信号通路调节大鼠再生肝的肝细胞增殖

发布时间：2018-05-04 12:26

本文选题：肝再生 + 肝细胞增殖　；参考：《中国生物化学与分子生物学报》2017年05期

【摘要】：生长激素(growth hormone,GH)信号通路对机体生长发育具有重要的调控作用。GH通过与特异性膜表面受体结合,启动下游一系列信号通路反应,进而调控细胞增殖、分化和迁移,防止细胞凋亡等。GH对细胞增殖的调控机制一直以来都是研究的热点,但部分肝切除(partial hepatectomy,PH)后,生长激素相关的信号通路是否会活化,调控相关基因的表达,从而促进肝实质细胞增殖,尚未见报道。本文以percoll密度梯度离心结合磁珠分离的大鼠再生肝的肝细胞为材料,采用Rat Genome 230 2.0芯片与生物信息学相结合的方法,研究GH信号通路对肝再生的调控作用。结果表明,大鼠再生肝的肝细胞中22种基因与GH信号通路相关,其中,Gh1、Jak3、Stat3等14种基因表达上调,Irs3、Ghr、Mras等8种基因表达下调。谱函数(Et)分析基因表达变化预示的细胞增殖活动和信号转导活性表明,GH信号通路的信号传导活性在大鼠肝再生的2~72 h强于对照,所调节的肝细胞增殖活动在6~72 h也强于对照。综上所述,GH信号通路促进大鼠再生肝的肝细胞增殖。
[Abstract]:Growth hormone growth hormone (GH) signaling pathway plays an important role in regulating the growth and development of the body. GH activates a series of downstream signal pathways by binding to the specific membrane surface receptor, thereby regulating cell proliferation, differentiation and migration. The regulation mechanism of GH on cell proliferation has been a hot topic for a long time, but after partial hepatectomy (PHH), whether the growth hormone related signaling pathway will be activated and the expression of related genes can be regulated. Thus promoting the proliferation of hepatic parenchyma cells has not been reported. Using percoll density gradient centrifugation and magnetic beads as materials, the regulation of GH signaling pathway on liver regeneration was studied by using Rat Genome 230 2.0 chip and bioinformatics. The results showed that 22 genes were related to GH signaling pathway in rat regenerative liver cells, and 14 genes, such as Gh1 / Jak3Stat3, up-regulated the expression of IRS3Ghr-MRas and down-regulated the expression of 8 genes. The cell proliferation and signal transduction activity predicted by the changes of gene expression showed that the signal transduction activity of GH signaling pathway in rat liver regeneration at 2 h was higher than that in the control group, and the regulated hepatocyte proliferation activity at 672 h was stronger than that in the control group. To sum up, the GH signaling pathway promotes the proliferation of hepatocytes in rat regenerated liver.
【作者单位】：河南师范大学生命科学学院河南省-科技部共建细胞分化调控国家重点实验培育基地;
【基金】：河南省自然科学基金(No.142300413212)资助~~
【分类号】：R575

【相似文献】