紫茉莉对酒精性肝损伤大鼠ALT、AST、T-SOD影响的研究

  本文关键词：紫茉莉对酒精性肝损伤大鼠ALT、AST、T-SOD影响的研究，由笔耕文化传播整理发布。

        目的通过观察紫茉莉根醇提物对酒精性肝损伤大鼠ALT、AST、T-SOD的影响，探讨紫茉莉对大鼠酒精性肝损伤的保护作用。方法SD大鼠40只，雌雄各半，随机分为3组：正常对照组10只、模型对照组及紫茉莉处理组各15只。正常组大鼠以蒸馏水灌胃，其余两组大鼠均以白酒灌胃，连续灌胃80天，每天1次。第51天开始，正常组大鼠以蒸馏水灌胃，其余两组大鼠均以相应药物灌胃，连续灌胃30天，每天1次。第80天末次给药后，禁食不禁水12h，摘眼球取血，处死大鼠取肝脏。测定血清中谷丙转氨酶(ALT)活性、谷草转氨酶(AST)活性及肝组织中总超氧化物歧化酶(T-SOD)活性；计算肝脏指数；观察肝组织病理变化。结果1、血清谷丙转氨酶(ALT)检测结果：与正常组比较，模型组和紫茉莉组大鼠血清中ALT活性均显著升高（P <0.01），表明两组大鼠肝脏均有不同程度损伤。与模型组比较，紫茉莉组大鼠血清中ALT活性显著降低（P <0.01），表明紫茉莉组大鼠肝脏损伤程度有减轻。2、血清谷草转氨酶(AST)检测结果：与正常组比较，模型组和紫茉莉组大鼠血清中AST活性均显著升高（P <0.01），，表明两组大鼠肝脏均有不同程度损伤。与模型组比较，紫茉莉组大鼠血清中AST活性显著降低（P <0.05），表明紫茉莉组大鼠肝脏损伤程度有减轻。3、肝组织中总超氧化物歧化酶(T-SOD)检测结果：与正常组比较，模型组和紫茉莉组大鼠肝组织中T-SOD活性均显著降低（P <0.01），表明两组大鼠肝组织均有不同程度过氧化损伤。与模型组比较，紫茉莉组大鼠肝组织中T-SOD活性显著升高（P <0.05），表明紫茉莉组大鼠肝组织过氧化损伤程度有减轻。4、肝脏指数检测结果：与正常组比较，模型组和紫茉莉组大鼠肝脏指数均显著增大（P <0.01），表明两组大鼠肝脏均有不同程度肿大。与模型组比较，紫茉莉组大鼠肝脏指数显著减小（P <0.05），表明紫茉莉组大鼠肝脏肿大程度有减小。5、肝组织病理变化：正常组肝组织结构正常；模型组肝细胞水样变性（水肿）伴灶状坏死；紫茉莉组与模型组比较，肝细胞水肿减轻，坏死减少，肝组织的形态结构显著改善。结论紫茉莉对大鼠酒精性肝损伤具有一定的保护作用，其机制可能与抑制过氧化反应，减轻酒精性肝病造成的肝细胞损伤有关。

    Objective: To observe the effects of Mirabilis Jalapa on ALT、AST、 T-SOD ofalcoholic liver injury rats and study the protecting effects on alcoholic liver injuryrats.Methods:40SD Rats were randomly divided to three groups, including model,Mirabilis Jalapa dealing and control groups. Hepatic injury models were establishedby intragastric administration of ethanol. The saline water and ethanol wereadministered intragastrically to the corresponding groups for80continuous days.From the51stday, the Mirabilis Jalapa was administered intragastriclly to accordinggroup for30continuous days. The blood sample and liver were collected in80thday. The concebtrations of aspartate aminotransferase(AST)、 alanineaminotransferase(ALT) and total superoxide dismutase(T-SOD) in serum wereassayed in according to the procedure described in kits. Then the hepatic indexes werecalculated and the pathological changes were observed.Results:1. The activities of ALT were improved in model and Mirabilis Jalapa groupcompared with control group. It indicated the liver injury model leading by ethanolwere established successfully. And the decline of the ALT level in Mirabilis Jalapagroup compared with model indicated the Mirabilis Jalapa can reduce the liver injuryleading by ethanol.2. The activities of AST were improved in model and Mirabilis Jalapa groupcompared with control group. It indicated the liver injury model leading by ethanolwere established successfully. And the decline of the AST level in Mirabilis Jalapagroup compared with model indicated the Mirabilis Jalapa can reduce the liver injuryleading by ethanol.3. The activities of T-SOD were decreased in model and Mirabilis Jalapa groupcompared with control group. It indicated the liver injury model leading by superoxide dismutase were established successfully. And the improve of the T-SODlevel in Mirabilis Jalapa group compared with model indicated the Mirabilis Jalapacan reduce the liver injury through inhibiting superoxide reaction.4. The hepatic index increased in model group and decreased in Mirabilis Jalapagroup compared with control. It indicated that the Mirabilis Jalapa can reduce thehypertrophy changing of liver leading by ethanol injury.5. The hydrophic degeneration and spotty necrosis of liver cells can be observed inmodel group and reduced in Mirabilis Jalapa group.Conclusion: The Mirabilis Jalapa can reduce the liver injury leading by ethanolthrough reducing the level of superoxide reaction to hepatic cells from ethanol.

紫茉莉对酒精性肝损伤大鼠ALT、AST、T-SOD影响的研究

目录3-4缩略词表4-5中文摘要5-7Abstract7-8前言9-12材料与方法12-18结果18-23讨论23-27结论27-28参考文献28-31综述31-38    参考文献36-38致谢38

  本文关键词：紫茉莉对酒精性肝损伤大鼠ALT、AST、T-SOD影响的研究，由笔耕文化传播整理发布。