超微七味白术散与酵母菌配伍治疗菌群失调腹泻的研究

发布时间：2018-05-18 08:54

本文选题：七味白术散 + 超微中药　；参考：《湖南中医药大学》2014年硕士论文

【摘要】：目的：研究超微七味白术散与酵母菌配伍治疗菌群失调腹泻小鼠的效果,明确七味白术散对肠道酵母菌生长的影响,为临床治疗和新药的开发与生产提供实验依据。方法：将70只昆明小鼠随机分为7组,以无菌水做正常组,灌胃头孢拉定和硫酸庆大霉素混合抗生素建立小鼠菌群失调腹泻模型后,分别灌胃给药七味白术散50%超微汤药、50%超微汤药+12.5%酵母、25%超微汤药+25%酵母、12.5%超微汤药+50%酵母及全量酵母菌,治疗结束后,通过分析小鼠体重的变化、免疫器官指数、肠道可培养菌群数及肠道内重要酶活性来评价超微七味白术散与酵母菌配伍治疗菌群失调腹泻小鼠的最佳配方。通过滤纸片法和比浊法测量七味白术散单味药及复方对肠道酵母菌生长的影响。结果：超微七味白术散和酵母菌配伍对菌群失调腹泻小鼠体重和脾脏无影响,各治疗组的胸腺指数均达到了正常水平(P0.05)；超微七味白术散和酵母菌均能促进双歧杆菌和乳酸菌的生长(P0.01),调节微生态平衡；50%超微汤药组和25%超微汤药+25%酵母组的大肠杆菌、细菌数恢复至正常水平(P0.05)。经治疗后,肠道内纤维素酶活性均恢复至正常组水平(P0.05)；50%超微汤药+12.5%酵母组和25%超微汤药+25%酵母组的淀粉酶活性恢复至正常(P0.05)；木聚糖酶、蛋白酶活性与超微饮片的浓度及酵母菌的量有一定的关系,但均未达到正常组水平(P0.05)。人参和甘草对肠道酵母菌具有抑制作用,人参的最低抑菌浓度为0.7936g/mL,甘草的最低抑菌浓度为0.3949g/mL；茯苓、白术、葛根对肠道酵母菌的生长具有促进作用,且在一定浓度范围内,药物浓度增高对酵母菌生长的促进作用增大；木香和藿香对肠道酵母菌未表现出明显的抑促作用。七味白术散复方在低浓度下对肠道酵母菌产生促进作用,七味白术散超微汤剂的作用效果要优于传统汤剂(P0.01)。结论：七味白术散25%超微汤药+25%酵母为最佳配伍,治疗菌群失调小鼠腹泻的效果与超微七味白术散50%量相当。人参和甘草对肠道酵母菌有抑制作用,茯苓、白术、葛根及七味白术散复方对肠道酵母菌的生长有促进作用,且超微药物的作用效果好于传统饮片
[Abstract]:Objective: to study the effect of Qiwei Baizhu Powder combined with yeast on the growth of intestinal yeast in mice with dysbacteriosis diarrhea, and to provide experimental basis for clinical treatment and the development and production of new drugs. Methods: 70 Kunming mice were randomly divided into 7 groups and treated with aseptic water as normal group. 50% Atractylodes powder 50% ultramicro soup drug 12.5% yeast 25% ultramicro soup drug 25% yeast 12.5% ultrafine soup drug 50% yeast and whole yeast after treatment, the changes of body weight and immune organ index of mice were analyzed after treatment. The number of culturable bacteria and the activity of important enzymes in intestinal tract were used to evaluate the best formula for the treatment of diarrhea mice with the combination of ultramicro Qiwei Baizhu powder and yeast. The effects of Qiwei Atractylodes powder and its compound on the growth of intestinal yeast were measured by filter paper method and turbidimetry. Results: the combination of Qiwei Baizhu Powder and yeast had no effect on the body weight and spleen of mice with dysbacteriosis and diarrhea. The thymus index of each treatment group reached the normal level (P0.05), the growth of Bifidobacterium and lactic acid bacteria was promoted by Qiwei Baizhu Powder and yeast, and the microecological balance was regulated by Escherichia coli in 50% ultrafine soup group and 25% yeast group. The number of bacteria returned to normal level (P0.05). After treatment, the activity of cellulase in the intestine returned to the normal level. The amylase activity of the 50% ultrafine soup group and 25% yeast group returned to normal P0.05, xylanase, P0.05%, xylanase, and xylanase. The activity of protease was related to the concentration of ultrafine slices and the quantity of yeast, but it did not reach the normal level of P0.05. Ginseng and Glycyrrhiza have inhibitory effect on intestinal yeast, the minimum inhibitory concentration of ginseng is 0.7936 g / mL, the minimum inhibitory concentration of licorice is 0.3949 g / mL; Poria cocos, Atractylodes macrocephala and Pueraria lobata have promoting effect on the growth of intestinal yeast, and within a certain concentration range, The increase of drug concentration increased the growth of Saccharomyces cerevisiae, but the growth of Saccharomyces cerevisiae was not significantly inhibited by Astragalus and patchouli. Qiwei Baizhu Powder compound can promote intestinal yeast at low concentration, and the effect of Qiwei Baizhu Powder ultrafine decoction is better than that of traditional decoction P0.01. Conclusion: Qiwei Baizhu Powder 25% Ultramicro decoction 25% yeast is the best compatibility and the effect of treating diarrhea in mice with dysbacteriosis is equal to 50% of Ultramicro Qiwei Atractylodes Powder. Ginseng and liquorice have inhibitory effect on intestinal yeast. Poria cocos, Atractylodes macrocephala, Pueraria lobata and Qiwei Baizhu Powder can promote the growth of intestinal yeast, and the effect of ultramicro medicine is better than that of traditional medicine.
【学位授予单位】：湖南中医药大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R574.62

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