替普瑞酮对胃上皮细胞增殖、迁移及凋亡的作用研究

发布时间：2018-05-19 20:45

本文选题：胃上皮细胞 + 替普瑞酮　；参考：《中南大学》2014年硕士论文

【摘要】：目的：观察替普瑞酮对人正常胃上皮细胞增殖、迁移及凋亡等生物学现象的作用方法： 1、体外培养人正常胃上皮细胞GES。 2.、用MTT法检测不同浓度替普瑞酮对胃上皮细胞增殖的作用及确定替普瑞酮最佳作用浓度。 3、用替普瑞酮最佳作用浓度培养胃正常上皮细胞,以无水乙醇溶剂组作为对照,采用TRANSWELL实验、划痕实验检测替普瑞酮对胃上皮细胞运动能力及迁移的作用。 4、以无水乙醇溶剂组作为对照,采用细胞流式分析方法检测替普瑞酮对胃上皮细胞凋亡的作用。结果： 1、替普瑞酮作用于胃上皮细胞24小时后,开始出现促进胃上皮细胞增殖的作用,从10uM-80uM随着浓度增加,促进作用越明显,而80uM-320uM随着浓度增加,促进作用无明显改变,同时,确定药物最佳作用浓度为80uM。 2、替普瑞酮处理胃上皮细胞24小时后,TRANSWELL小室膜下蓝染的细胞明显多于对照组,溶剂对照组的迁移率为1.328,加药组的迁移率为3.338,加药组迁移率明显高于对照组(P0.01),因此,加药后胃上皮细胞的迁移率明显增加。 3、替普瑞酮处理胃上皮细胞8小时后,加药组划痕区域细胞迁移率为0.54,对照组为0.38,加药组与对照组迁移率无明显差异(P0.05),而替普瑞酮作用24h后,加药组迁移率为1.00,对照组迁移率为0.72,加药组迁移率明显高于对照组(P0.05),因此,加药后胃上皮细胞的迁移率增加。 4、替普瑞酮处理胃上皮细胞48小时后,加药组与对照组相比,早期凋亡细胞及晚期凋亡细胞均明显减少,加药组细胞凋亡率为11.9%,对照组细胞凋亡率为24.54%,加药组细胞的凋亡率低于对照组(P0.05)。结论： 1、替普瑞酮能够促进人正常胃上皮细胞增殖。 2、替普瑞酮能够促进人正常胃上皮细胞迁移。 3、替普瑞酮能够抑制人正常胃上皮细胞凋亡。
[Abstract]:Objective: to observe the effects of tipranone on proliferation, migration and apoptosis of human normal gastric epithelial cells. Methods: 1. Human normal gastric epithelial cells were cultured in vitro. 2. The effects of different concentrations of tipranone on the proliferation of gastric epithelial cells were detected by MTT assay and the optimum concentration of tipranone was determined. 3. Normal gastric epithelial cells were cultured with the optimal concentration of tipranone. The effects of tipranone on the motility and migration of gastric epithelial cells were detected by TRANSWELL test and scratch test. 4. The effect of tipranone on apoptosis of gastric epithelial cells was detected by flow cytometry with anhydrous ethanol solvent as control group. Results: 1. After treated with tipranone for 24 hours, the proliferation of gastric epithelial cells began to be promoted. With the increase of 10uM-80uM concentration, the promoting effect was more obvious, but 80uM-320uM had no obvious change with the increase of concentration, at the same time, the effect of tipranone on the proliferation of gastric epithelial cells began to appear. The optimum concentration of the drug was determined to be 80 UM. 2. After treated with tipranone for 24 hours, the number of blue stained cells in the subventricular membrane of TRANSWELL was significantly higher than that in the control group, the mobility of the solvent control group was 1.328, the migration rate of the drug added group was 3.338, and the migration rate of the drug added group was significantly higher than that of the control group (P 0.01). The migration rate of gastric epithelial cells was significantly increased after administration. 3. After treated with tipranone for 8 hours, the cell migration rate of scratched area was 0.54 in the treatment group and 0.38 in the control group. There was no significant difference in the migration rate between the two groups (P 0.05). However, after 24 hours of treatment with tipranone, the migration rate of the cells in the scratch area was 0.54, and that in the control group was 0.38. The mobility of gastric epithelial cells was significantly higher in the admixture group than that in the control group (1.00 vs 0.72). (4) after treated with tipranone for 48 hours, the number of early apoptotic cells and late apoptotic cells in the treatment group was significantly lower than that in the control group. The apoptotic rate of the drug added group was 11.9% and that of the control group was 24.54%. The apoptosis rate of the drug added group was lower than that of the control group (P 0.05). Conclusion: 1. Tipranone can promote the proliferation of normal gastric epithelial cells. 2, tipranone can promote the migration of normal gastric epithelial cells. 3, tipranone can inhibit the apoptosis of normal gastric epithelial cells.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R57

【共引文献】