基于IPA分析自噬对大鼠肝再生中树突状细胞的调节作用

发布时间：2018-05-21 21:30

本文选题：肝再生 + 自噬　；参考：《遗传》2015年03期

【摘要】：为探讨自噬对大鼠肝再生中树突状细胞(Dendritic cells,DCs)的调节作用,文章通过Percoll密度梯度离心结合免疫磁珠分选分离大鼠DCs,Rat Genome 230 2.0芯片检测大鼠肝再生中自噬相关基因表达变化,利用IPA等软件分析自噬在DCs中的生理活动。结果表明,LC3、BECN1、ATG7和SQSTM1等关键基因在部分肝切除后不同恢复时间段有明显表达变化;芯片中对应的自噬相关基因为593个,其中210个基因发生了有意义的变化。比较分析自噬生理活动情况,发现自噬在再生早期和晚期阶段增强,增殖期减弱。与自噬相关的生理活动主要有RNA表达、RNA转录细胞分化和增殖,其中涉及的信号通路主要有PPARα/RXRα激活、急性期反应、TREM1信号通路、IL-6信号通路、IL-8信号通路和IL-1信号通路等,它们在肝再生阶段发生了不同程度的上调或下调。Cluster分析还发现,P53和AMPK信号参与调控DCs的自噬活动,在肝再生早期主要是AMPK信号,在肝再生末期P53和AMPK信号共同参与自噬的调节。以上研究结果说明DCs自噬可能在肝再生早期激活细胞免疫反应和后期清除DCs等方面发挥着重要作用。
[Abstract]:In order to investigate the regulation of autophagy on Dendritic cells (DCs) in rat liver regeneration, the expression of autophagy related genes in rat liver regeneration was detected by Percoll density gradient centrifugation combined with immunomagnetic beads, and Rat Genome 2302 chip was used to detect the changes of autophagy related gene expression in rat liver regeneration. The physiological activities of autophagy in DCs were analyzed by IPA and other software. The results showed that the key genes, such as LC3, BECN1, ATG7 and SQSTM1, were obviously expressed in different recovery periods after partial hepatectomy. The corresponding autophagy related genes in the chip were 593, of which 210 genes had significant changes. The autophagy was compared and analyzed, and the autophagy was enhanced in the early and late stages of regeneration. The physiological activities related to autophagy mainly include RNA expression, RNA transcriptional cell differentiation and proliferation. The signal pathways involved mainly include PPAR alpha /RXR alpha activation, acute phase reaction, TREM1 signaling pathway, IL-6 signaling pathway, IL-8 signaling pathway and IL-1 signaling pathway, which have been up or down to varying degrees in the stage of liver regeneration. The Cluster analysis also found that P53 and AMPK signals participate in the autophagy regulation of DCs, which is mainly the AMPK signal in the early stage of liver regeneration. The P53 and AMPK signals are involved in the regulation of autophagy at the end of the stage of liver regeneration. The above results suggest that autophagy may play an important role in activating cell immune response and clearing DCs in the early stage of liver regeneration.
【作者单位】：河南师范大学河南省生物工程重点实验室;河南师范大学河南省-科技部共建细胞分化调控国家重点实验室培育基地;河南师范大学生命科学学院;
【基金】：河南省重大科技攻关项目(编号:111100910600) 河南师范大学博士启动课题(编号:qd14175) 河南师范大学青年科学基金项目资助
【分类号】：R575

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