石家庄地区乙肝病毒基因型分布及其与疾病进展关联研究

发布时间：2018-05-25 00:10

本文选题：乙型肝炎病毒 + 基因型　；参考：《现代预防医学》2017年07期

【摘要】：目的明确石家庄地区乙肝基因型/亚型分布特征,探索其与乙肝感染后疾病进展的相关性。方法采用型特异性引物多重聚合酶链反应(PCR)方法对270例乙肝表面抗原(HBsAg)阳性慢性乙肝、肝硬化和原发性肝癌患者血清标本进行HBV基因型/亚型检测,应用SPSS16.0进行统计描述和检验。结果基因型检测成功样本共233例,其中,C型占81.11%(189/233),B型占18.03%(42/233),D型占0.86%(2/233),基因亚型检测结果为:C2亚型占75.10%(175/233),B2亚型占16.74%(39/233),C1亚型占6.01%(14/233),B1亚型占1.29%(3/233);原发性肝癌、慢性乙肝患者中C基因型比例显著高于肝硬化(P=0.013,P=0.026);C基因型HBV感染者相对B型具有更高的HBe Ag阳性率和HBV DNA滴度(P=0.012,P=0.002);相对B型,C型HBV患者更易进展为肝硬化失代偿期(P=0.006),且具有显著更高的患癌风险(P=0.029)。结论石家庄地区流行HBV以C型和B型为主,其中C2、B2为主要亚型,HBV C型感染者更容易进展为失代偿肝硬化,发生肝癌的风险更高,但是需要更大样本量研究进行验证。
[Abstract]:Objective to determine the distribution of hepatitis B genotype / subtype in Shijiazhuang area and to explore its correlation with the progression of hepatitis B infection. Methods HBV genotypes / subtypes were detected by type specific primer polymerase chain reaction (PCR) in 270 serum samples from patients with chronic hepatitis B, liver cirrhosis and primary liver cancer. Use SPSS16.0 to describe and test statistics. Results there were 233 successful genotypic samples, of which 81.11c / 81.113B / 18.03 / 42 / 233C / D / 0.86 / 233C, and 75.101055 / 233B2 / 75.101055 / 233B2 / 16.7474 / 393233C _ 1 / 6.01p / 233mb _ 1 / 1.29 / 233C ~ (3 / 23); Primary liver cancer, respectively, = 1.29% / 2333 / 23 ~ (3 / 23); Primary liver cancer,% C _ 2 = 175.75% / 233B ~ (-1) = 16.74 / 39 / 23 ~ 3 / C ~ 1 = 6.01% / 23 / 23 ~ (-1). The ratio of genotype C to genotype C was significantly higher in patients with chronic hepatitis B than that in patients with liver cirrhosis. The positive rate of HBe Ag and the titer of HBV DNA in patients with HBV were higher than those in patients with type B HBV, and the patients with type B HBV were more likely to progress to the stage of decompensation of liver cirrhosis (P0.006). And the risk of cancer was significantly higher than that of P0. 029. Conclusion the prevalence of HBV in Shijiazhuang is mainly of type C and B, in which type C infection of C2B 2 is more likely to develop into decompensated cirrhosis, and the risk of liver cancer is higher, but larger sample size is needed to verify it.
【作者单位】：白求恩医务士官学校预防医学教研室;白求恩国际和平医院检验科;
【基金】：河北省自然科学基金(H2015509001)
【分类号】：R512.62

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