抗氧化治疗对急性重症胰腺炎大鼠的效果及JNK活化的影响

发布时间：2018-05-25 01:03

本文选题：抗氧化 + N-乙酰半胱氨酸　；参考：《广东医学》2015年14期

【摘要】：目的探讨抗氧化剂N-乙酰半胱氨酸对急性重症胰腺炎大鼠的疗效及JNK信号通路活化的影响。方法选择清洁级C57BL/6雄性SD大鼠56只,随机分为空白对照组8只,开腹后仅翻动胰腺,不做任何处理,然后立即关腹;胰腺炎组24只,采用10%的L-精氨酸腹腔注射法诱导大鼠急性重症胰腺炎模型;治疗组24只,于模型制备前30 min内腹腔注射5%的N-乙酰半胱氨酸(200 mg/kg)进行预处理,然后再进行急性重症胰腺炎大鼠模型制备;胰腺炎组和治疗组选取3、6、12 h 3个时间点各8只分为3个小组,观察各组大鼠血清胰淀粉酶、病理学改变、组织中磷酸化c-jun蛋白水平、丙二醛含量TNF-α、IL-6的变化。结果胰腺炎组大鼠各时间点的各项指标明显高于空白对照组;治疗组大鼠3、6 h血浆IL-6水平明显低于胰腺炎组,6、12 h胰腺组织病理学评分均明显低于胰腺炎组,其余各指标明显低于胰腺炎组(P0.05)。结论急性重症胰腺炎大鼠血清胰淀粉酶、病理学及相关指标均发生了明显的改变,N-乙酰半胱氨酸通过清除氧自由基、减少TNF-α和IL-6的产生等机制对急性重症胰腺炎大鼠发挥治疗和保护作用,还通过改变组织中磷酸化c-jun蛋白水平来影响JNK信号通路的活化。
[Abstract]:Objective to investigate the effect of antioxidant N-acetylcysteine on acute severe pancreatitis rats and the activation of JNK signaling pathway. Methods Fifty-six C57BL/6 male SD rats of clean grade were randomly divided into control group (n = 8). Acute severe pancreatitis in rats was induced by 10% L-arginine intraperitoneal injection, and 24 rats in the treatment group were pretreated with 5% N-acetylcysteine 200 mg / kg 30 min before the preparation of the model. The rats in the acute severe pancreatitis group and the treatment group were divided into 3 groups at each time point of 6 hours and 12 hours. The serum amylase, pathological changes and phosphorylated c-jun protein levels in the tissues were observed in each group. Changes of malondialdehyde (MDA) content in TNF- 伪 and IL-6. Results the indexes of each time point in the pancreatitis group were significantly higher than those in the blank control group, and the plasma IL-6 level in the treatment group was significantly lower than that in the pancreatitis group at 612 h, and the plasma IL-6 level in the treatment group was significantly lower than that in the pancreatitis group at 612 h. The other indexes were significantly lower than those in the pancreatitis group (P 0.05). Conclusion the changes of serum amylase, histopathology and related indexes in rats with severe acute pancreatitis have been observed by scavenging oxygen free radicals with N-acetylcysteine. The mechanism of decreasing the production of TNF- 伪 and IL-6 plays a therapeutic and protective role in rats with severe acute pancreatitis. It also affects the activation of JNK signaling pathway by changing the level of phosphorylated c-jun protein in the tissue.
【作者单位】：浙江省立同德医院普外科;
【基金】：浙江省医药卫生科技计划项目(编号:2012KYB041)
【分类号】：R576

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