慢性HBV感染不同临床转归患者外周血NK细胞PD-1的表达

发布时间：2018-06-10 00:56

本文选题：程序性凋亡因子 + 自然杀伤细胞　；参考：《广东医学》2016年S1期

【摘要】：目的比较慢性乙型肝炎病毒(hepatitis B virus,HBV)感染不同临床转归患者外周血自然杀伤(NK)细胞程序性凋亡因子-1(programmed death-1,PD-1)的表达情况。方法流式细胞术检测30例健康者(对照组)、17例慢性HBV携带者(携带组)、53例慢性肝炎者(慢性肝炎组)、18例肝炎肝硬化患者(肝硬化组)、16例乙肝相关原发性肝癌者(肝癌组)外周血NK细胞PD-1表达率,并进行比较。结果对照组NK细胞PD-1表达率[(8.2±2.0)%]明显低于携带组[(15.9±5.6)%]、肝硬化组[(14.8±6.8)%]和肝癌组[(14.8±4.1)%],差异有统计学意义(P=0.000)。对照组与慢性肝炎组[(10.6±4.5)%]相比,差异无统计学意义(P=0.258)。4组慢性HBV感染不同临床转归组NK细胞PD-1表达率比较,仅携带组NK细胞PD-1表达率高于慢性肝炎组,差异有统计学意义(P=0.001)。结论 NK细胞高表达PD-1可能影响机体对HBV感染的清除。
[Abstract]:Objective to compare the expression of programmed apoptotic factor-1 (PLF-1) in peripheral blood of patients with chronic hepatitis B virus (HBV) infection in peripheral blood of patients with natural killer NKK (NK) and to compare the expression of programmed death-1 (PD-1) in peripheral blood of patients with chronic hepatitis B virus (HBV) infection. Methods flow cytometry was performed in 30 healthy subjects (control group, 17 patients with chronic HBV carriers) and 53 patients with chronic hepatitis (chronic hepatitis group, 18 patients with liver cirrhosis and 16 patients with primary liver cancer associated with hepatitis B). The expression rate of PD-1 in peripheral blood NK cells, And the comparison was made. Results the expression rate of PD-1 on NK cells in the control group was significantly lower than that in the carrying group [15.9 卤5.6%], cirrhosis group (14.8 卤6.8%) and liver cancer group (14.8 卤4.1%). The difference was statistically significant. There was no significant difference between the control group and the chronic hepatitis group (10.6 卤4.5%). There was no significant difference in the expression rate of NK cell PD-1 between the control group and the chronic hepatitis group (P < 0. 258.4), but the expression rate of NK cell PD-1 in the carrier group was higher than that in the chronic hepatitis group (P 0. 001). Conclusion the high expression of PD-1 in NK cells may affect the clearance of HBV infection.
【作者单位】：广东省汕头市中心医院感染科;广东省汕头市中心医院检验科;
【基金】：广东省医学科研基金立项课题(编号:B2014375)
【分类号】：R512.62

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