芒果苷改善高果糖所致HepG2细胞内甘油三酯沉积机制研究

发布时间：2018-06-10 09:12

本文选题：芒果苷 + 果糖　；参考：《第三军医大学学报》2015年20期

【摘要】：目的研究芒果苷改善高果糖诱导的Hep G2细胞内甘油三酯沉积的可能机制。方法低糖(1 g/L葡萄糖)条件下培养的Hep G2细胞分为对照组(1 g/L葡萄糖,无果糖)、果糖组(1 g/L葡萄糖+30 mmol/L果糖)、果糖+芒果苷组(同时加入葡萄糖浓度1 g/L+30 mmol/L果糖+不同剂量的芒果苷,使其药物终浓度分别为6.25、12.5、25、50μmol/L),药物干预24 h之后,油红O染色观察细胞内脂滴的沉积情况,酶法测定细胞内甘油三酯(TG)的含量,Real-time PCR检测碳水化合物反应元件结合蛋白(ChREBP)、固醇调节元件结合蛋白1c(SREBP-1c)、肝型丙酮酸激酶(LPK)、二酯酰甘油酰基转移酶2(DGAT-2)mRNA表达的变化。结果油红O染色结果显示,与对照组相比,果糖组的Hep G2细胞脂滴显著增多,细胞内TG含量也明显升高(P0.05)。与果糖组对比,低剂量的芒果苷对果糖导致的细胞内脂滴的沉积无影响,较高剂量的芒果苷(25μmol/L和50μmol/L)使细胞内的脂滴数量明显减少,细胞内TG含量显著降低(P0.05),以50μmol/L的芒果苷干预效果最好。Real-time PCR结果显示,与对照组相比,果糖组Hep G2细胞ChREBP、SREBP-1c、LPK、DGAT-2 mRNA明显升高,50μmol/L芒果苷能够下调Hep G2细胞ChREBP、LPK、DGAT-2 mRNA的高表达(P0.05),但对SREBP-1c mRNA表达影响不明显。结论芒果苷能够改善高果糖诱导的Hep G2细胞内TG的沉积,可能与抑制脂质合成相关基因ChREBP、LPK、DGAT-2 mRNA的表达密切相关。
[Abstract]:Objective to study the possible mechanism of mangiferin in improving triglyceride deposition in Hep G2 cells induced by high fructose. Methods HepG2 cells cultured with low glucose (1 g / L glucose) were divided into control group (1 g / L glucose). Without fructose, the fructose group with 1 g / L glucose 30 mmol / L fructose, fructose mangiferin group (1 g / L 30 mmol / L fructose with different doses of mangiferin was added at the same time, the final drug concentration was 6.25% 12.55% 25 渭 mol / L, respectively. After 24 hours of drug intervention, Oil red O staining was used to observe the deposition of lipid droplets in cells. Real-time PCR was used to detect the expression of carbohydrate response element binding protein (CHREBPN), steroid regulatory element binding protein (1cctbbp1), hepatic pyruvate kinase (LPKK) and diester acylglyceryl transferase (2DGAT-2). Results the results of oil red O staining showed that the lipid droplets of Hep G2 cells in fructose group were significantly higher than those of the control group, and the content of TG in the cells was also significantly higher than that in the control group. Compared with fructose group, low dose mangiferin had no effect on lipid droplet deposition in cells induced by fructose, and high dose mangiferin 25 渭 mol / L and 50 渭 mol / L) significantly reduced the number of lipid droplets in cells. The content of TG in cells decreased significantly (P 0.05). Compared with the control group, 50 渭 mol / L mangiferin had the best effect on real-time PCR. Fructose treated Hep G2 cell line ChREBPnSREBP-1 / LPKP-1 / LPKDGAT-2mRNA increased by 50 渭 mol / L mangiferin could down-regulate the high expression of P0.05mRNA in Hep G2 cells, but had no significant effect on the expression of SREBP-1c mRNA. Conclusion mangiferin can improve the deposition of TG in Hep G2 cells induced by high fructose, which may be related to the inhibition of the expression of LPKN DGAT-2 mRNA in Hep G2 cells induced by high fructose.
【作者单位】：重庆医科大学基础医学院干细胞与组织工程研究室;重庆医科大学生命科学院脂糖代谢重点实验室;重庆医科大学中医药学院中医药研究室;
【基金】：国家自然科学基金面上项目(81374033)~~
【分类号】：R575.5

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