血清IL-12B表达与溃疡性结肠炎疾病活动性及其黏膜愈合关系的研究

发布时间：2018-06-12 07:26

本文选题：溃疡性结肠炎 + IL-12B　；参考：《青岛大学》2017年硕士论文

【摘要】：研究背景:溃疡性结肠炎(ulcerative colitis-UC)是炎症性肠病的一种,为非特异性慢性炎症性疾病,目前病因尚未明确。虽然目前一些无创、方便的生物标志物检测UC具有一定价值,但敏感性和特异性有限,亦取代不了内镜评价的需要。内镜因其经济因素及操作的风险性,尚不能成为溃疡性结肠炎的普查手段。有研究表明血清IL-12B的表达与UC的活动性具有相关性,血清IL-12B有望成为预测UC活动性及预后的有效标志物。研究目的:测定UC患者临床及内镜下不同活动期的血清IL-12B的表达水平,并进行比较,确定血清IL-12B表达与UC活动性及其黏膜愈合的相关性,评价血清IL-12B作为一种诊断溃疡性结肠炎的生物标记物的可能性。研究方法:收集80例溃疡性结肠炎住院患者及20例健康对照者的临床信息、血清及结肠镜检查结果,均来自青岛市市立医院,血清IL-12B浓度采用酶联免疫吸附法(ELISA)测定,溃疡性结肠炎临床活动度由炎症性肠病Montreal分类评估,溃疡性结肠炎内镜下疾病活动度以Mayo内镜评分为标准。并将溃疡性结肠炎患者临床和内镜下血清IL-12B浓度联合进行评估。P0.05认为有统计学意义。研究结果:溃疡性结肠炎患者血清IL-12B水平明显高于对照组(2.75pg/ml,1.43pg/ml,P0.05)。临床活动期明显高于临床缓解期(3.0pg/ml,1.05pg/ml,P0.05),差异有统计学意义;内镜下活动期高于缓解期(1.88pg/ml,1.74pg/ml,P0.05),差异有统计学意义;疾病变化病程中IL-12B水平变化采用配对t检验,Spearman相关分析分别检测IL-12B表达水平与临床及内镜下疾病严重程度,均呈正相关(r=0.90,r=0.95,P0.05)。研究结论:血清IL-12B水平与溃疡性结肠炎疾病活动性及黏膜愈合具有相关性(P0.05),血清IL-12B水平随溃疡性结肠炎严重程度增加而增加(P0.05)。表明血清IL-12B可以成为UC患者疾病活动性及黏膜愈合评估的潜在生物标志物。
[Abstract]:Background: ulcerative colitis-UCis is a kind of inflammatory bowel disease, which is nonspecific chronic inflammatory disease. Although some noninvasive and convenient biomarker detection of UC has some value, but the sensitivity and specificity is limited, it can not replace the need of endoscopic evaluation. Because of its economic factors and operational risk, endoscopy can not be used as a general method for ulcerative colitis. Some studies have shown that the expression of serum IL-12B is correlated with the activity of UC, and serum IL-12B may be an effective marker for predicting UC activity and prognosis. Objective: to determine the relationship between serum IL-12B expression and UC activity and mucosal healing in patients with UC. To evaluate the possibility of serum IL-12 B as a biomarker for diagnosis of ulcerative colitis. Methods: the clinical information of 80 patients with ulcerative colitis and 20 healthy controls were collected. The results of serum and colonoscopy were collected from Qingdao City Hospital. The serum IL-12B concentration was determined by enzyme linked immunosorbent assay (Elisa). The clinical activity of ulcerative colitis was evaluated by Montreal classification of inflammatory bowel disease. The clinical and endoscopic serum IL-12B levels in patients with ulcerative colitis were evaluated. Results: the serum levels of IL-12B in patients with ulcerative colitis were significantly higher than those in the control group (2.75 PG / ml 1.43 PG / ml P 0.05). The difference was statistically significant in the active stage than that in the remission stage (3.0pg / ml, 1.05pg / ml, P 0.05N), and in the active stage under endoscopy was 1.88pg / ml 1.74pg / ml, the difference was statistically significant, compared with that in the remission stage (1.88pg / ml, 1.74pg / ml, P 0.05g / L). The expression of IL-12B was detected by paired t-test and Spearman correlation analysis. The expression of IL-12B was positively correlated with the clinical and endoscopic disease severity. Conclusion: the level of serum IL-12B is correlated with the activity of ulcerative colitis and mucosal healing. The level of serum IL-12B increases with the severity of ulcerative colitis. Serum IL-12 B may be a potential biomarker for the assessment of disease activity and mucosal healing in UC patients.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R574.62

【参考文献】