p38信号通路对急性重症胰腺炎大鼠大脑皮层COX-2表达的影响

发布时间：2018-06-13 09:19

本文选题：胰腺炎 + 脑损伤　；参考：《山东医药》2015年34期

【摘要】：目的观察p38信号通路对急性重症胰腺炎(SAP)大鼠大脑皮层环氧合酶2(COX-2)表达的影响。方法将45只大鼠随机分为模型组、抑制剂组、假手术组各15只。抑制剂组、模型组分别将5%牛磺胆酸钠经胰胆管逆行注入胰腺制备SAP大鼠模型,造模后5 min于大鼠尾静脉分别注射p38信号通路抑制剂SB203580及等量生理盐水;假手术组开腹后翻动胰腺数次立即缝合腹壁,尾静脉注射等量生理盐水。各组于造模后24 h断头取脑,采用Western blot法检测大脑皮层COX-2和磷酸化p38(p-p38),计算其平均光密度(IOD)值。结果抑制剂组pp38、COX-2蛋白表达的IOD值分别为7.5±0.9、7.5±1.1,模型组分别为18.3±1.3、13.3±3.0,假手术组分别为2.1±0.3、5.3±1.0;三组间两两比较,P均0.05。相关分析显示,p-p38与COX-2蛋白表达呈正相关(r=0.661,P0.05)。结论 p38信号通路具有调控SAP大鼠皮层COX-2表达的作用,抑制该通路可下调COX-2表达。
[Abstract]:Objective to observe the effect of p38 signal pathway on the expression of cyclooxygenase 2 (COX-2) in rat cerebral cortex with severe acute pancreatitis (SAP). Methods 45 rats were randomly divided into three groups: model group, inhibitor group and sham operation group. In the inhibitor group and the model group, 5% sodium taurocholate was retrograde injected into the pancreas through the pancreatic duct to make the SAP rat model. 5 min after the establishment of the model, SB203580, a p38 signal pathway inhibitor, and the same amount of normal saline were injected into the tail vein of the rats respectively. In the sham operation group, the pancreas was turned over several times and the abdominal wall was sutured immediately, and the same amount of normal saline was injected into the caudal vein. The cerebral cortex COX-2 and phosphorylated p38 p-p38 were detected by Western blot method, and the mean optical density (IOD) was calculated. Results the IOD of COX-2 protein expression in inhibitor group was 7.5 卤0.9 卤7.5 卤1.1, in model group was 18.3 卤1.3 卤3.0, and in sham-operated group was 2.1 卤0.35.3.The difference among the three groups was 0.05. Correlation analysis showed that there was a positive correlation between p-p38 and COX-2 protein expression. Conclusion p38 signaling pathway can regulate the expression of COX-2 in the cortex of SAP rats and down-regulate the expression of COX-2 in SAP rats.
【作者单位】：唐山市丰润区人民医院;河北联合大学解剖学教研室;
【基金】：河北省唐山市科学技术研究与发展计划项目(111302069a)
【分类号】：R576

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