功能性胃肠病粪便及肠黏膜菌群与ZO-1蛋白的相关性研究

发布时间：2018-06-13 10:18

本文选题：肠腔菌群 + 肠粘膜菌群　；参考：《山西医科大学》2017年硕士论文

【摘要】：目的:探讨功能性胃肠病(functional gastrointestinal disorders,FGIDs)患者粪便菌群和肠黏膜菌群的差异性以及二者与肠粘膜中紧密连接蛋白ZO-1的相关性。方法:通过结肠镜检查收集32例功能性胃肠病患者的降结肠黏膜组织以及肠道清洁前粪便标本,采用高通量测序及实时荧光定量PCR技术检测粪便中以及肠黏膜中拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)、疣微菌门(Verrucomicrobia)、放线菌门(Actinobacteria)、蓝藻门(Cyanobacteria)、Candidate、梭杆菌门(Fusobacteria)8种菌所占比例,粪便中及肠黏膜定植的拟杆菌门、厚壁菌门、乳酸菌、肠球菌、肠杆菌5种菌的DNA含量与结肠黏膜组织中紧密连接蛋白ZO-1的m RNA的表达情况,比较粪便菌群与肠黏膜菌群的差异性,并分析粪便及肠黏膜优势菌群与紧密连接蛋白ZO-1表达的相关性。结果:肠道粪便菌群与肠黏膜菌群不同,粪便菌群较肠粘膜菌群多样性(Shannon指数)增加,肠道粪便组为=2.901±0.54,肠粘膜组为=2.29±1.24,P=0.01200.05,有统计学意义。在门水平上,粪便菌中以拟杆菌门及厚壁菌门数量最多,占主要成分;而在肠黏膜组织中变形菌门数量最多。肠黏膜拟杆菌含量与ZO-1mRNA呈正相关(r=0.378;P0.05);而粪便拟杆菌与黏膜组织ZO-1mRNA表达呈负相关(r=-0.42,P0.05);组织中乳酸菌和肠杆菌均与ZO-1呈正相关(r=0.53,P0.01;r=0.355,P0.05)。结论:肠道菌群包括粪便菌群和粘膜菌群,粪便菌群与粘膜菌群存在差异性,且粪便和黏膜的同一菌群与ZO-1的相关性不同甚至相悖。
[Abstract]:Objective: to investigate the difference between fecal flora and intestinal mucosal flora in patients with functional gastrointestinal disordersFGIDsand the correlation between them and ZO-1 in intestinal mucosa. Methods: the specimens of descending colon mucosa and feces before intestinal cleaning were collected from 32 patients with functional gastrointestinal disease by colonoscopy. High throughput sequencing and real-time fluorescent quantitative PCR were used to detect eight species of Bacteroides, Firmicuteschus, Proteobacteria, Vertinobacteria, Actinobacteria, Cyanobacteria, and Fusobacteriae in feces and intestinal mucosa. DNA content in fecal and intestinal mucosal colonization of Bacteroides phylum, Mycobacterium thuringiensis, Lactobacillus, Enterococcus and Enterobacter spp., and the expression of tight junction protein ZO-1 mRNA in colonic mucosa. The differences between fecal microflora and intestinal mucosal flora were compared, and the correlation between fecal and intestinal mucosal dominant flora and the expression of tight junction protein ZO-1 was analyzed. Results: the faecal microflora was different from that of intestinal mucosal flora, and the diversity of fecal microflora was higher than that of intestinal mucosal microflora (Shannon index). The intestinal fecal microflora was 2.901 卤0.54, and the intestinal mucosal group was 2.29 卤1.24Pu 0.01200.05. there was a significant difference between the fecal microflora and the intestinal mucosal group (P < 0.05). At the portal level, the number of faecal bacteria was the largest in Bacteroides sp. And the phylum of Thinacea was the most, and the number of Proteus in intestinal mucosa was the most. There was a positive correlation between ZO-1 mRNA and ZO-1 mRNA, but a negative correlation between faecal bacillus and ZO-1 mRNA expression, and a positive correlation between Lactic acid bacteria and Enterobacter spp. And ZO-1, 0.355P0.05and 0.373P0.01P0.05P 0.05. the results showed that there was a positive correlation between ZO-1mRNA and ZO-1mRNA, but there was a negative correlation between the expression of ZO-1mRNA and the expression of ZO-1mRNA, and the positive correlation between Lactobacillus and Enterobacter spp. Conclusion: the intestinal flora includes faecal flora and mucosal flora. There are differences between fecal flora and mucosal flora, and the correlation between the same faecal and mucosal flora and ZO-1 is different or even contrary.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R57

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